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• W1873202260 endingPage "1799" @default.
• W1873202260 startingPage "1795" @default.
• W1873202260 abstract "The major open reading frame of banana bunchy top virus (BBTV) DNA-1 encodes a putative replication initiation protein (Rep). In vitro, a fusion protein of BBTV Rep linked to a maltose-binding protein exhibited both site-specific nicking and joining activities. These activities were dependent on the presence of Mg2 or Mn2 , but did not require ATP. The fusion protein specifically cleaved ssDNA between bases 7 and 8 of a conserved nonanucleotide loop sequence which is present in the virion-strand of the stem-loop common region of each BBTV component. During this reaction, the fusion protein became covalently attached to the 5′ end of the 3 cleavage product. After the nicking reactions, the fusion protein was also capable of catalysing the joining of two nicked ssDNA fragments in a site-specific manner. Based on these activities, BBTV Rep would appear to be very similar to the Rep proteins of the geminiviruses." @default.
• W1873202260 created "2016-06-24" @default.
• W1873202260 creator A5017433135 @default.
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• W1873202260 date "1997-07-01" @default.
• W1873202260 modified "2023-10-14" @default.
• W1873202260 title "Nicking and joining activity of banana bunchy top virus replication protein in vitro." @default.
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• W1873202260 doi "https://doi.org/10.1099/0022-1317-78-7-1795" @default.
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