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- W1873662004 abstract "This is a simple, rapid, sensitive method for routine quantification of the polyamine putrescine (1,4-diaminobutane) in cerebrospinal fluid. Sample preparation involves protein precipitation, acid hydrolysis for 18 h (if total putrescine is to be measured), pre-column derivatization with o-phthalaldehyde, and extraction into acetonitrile. The derivative is separated and quantified by high-performance liquid chromatography on a reversed-phase C18 radial-compression column. A single chromatographic run takes less than 18 min. Putrescine concentrations as low as 50 nmol/L in cerebrospinal fluid can be detected." @default.
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- W1873662004 date "1984-07-01" @default.
- W1873662004 modified "2023-09-24" @default.
- W1873662004 title "Free and total putrescine in cerebrospinal fluid quantified by reversed-phase liquid chromatography." @default.
- W1873662004 doi "https://doi.org/10.1093/clinchem/30.7.1243" @default.
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