FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1874277632> ?p ?o ?g. }

• W1874277632 endingPage "3187" @default.
• W1874277632 startingPage "3177" @default.
• W1874277632 abstract "Methanobacterium thermoautotrophicum delta H and Marburg were adapted to grow in medium containing up to 0.65 M NaCl. From 0.01 to 0.5 M NaCl, there was a lag before cell growth which increased with increasing external NaCl. The effect of NaCl on methane production was not significant once the cells began to grow. Intracellular solutes were monitored by nuclear magnetic resonance (NMR) spectroscopy as a function of osmotic stress. In the delta H strain, the major intracellular small organic solutes, cyclic-2,3-diphosphoglycerate and glutamate, increased at most twofold between 0.01 and 0.4 M NaCl and decreased when the external NaCl was 0.5 M. M. thermoautotrophicum Marburg similarly showed a decrease in solute (cyclic-2,3-diphosphoglycerate, 1,3,4,6-tetracarboxyhexane, and L-alpha-glutamate) concentrations for cells grown in medium containing > 0.5 M NaCl. At 0.65 M NaCl, a new organic solute, which was visible in only trace amounts at the lower NaCl concentrations, became the dominant solute. Intracellular potassium in the delta H strain, detected by atomic absorption and 39K NMR, was roughly constant between 0.01 and 0.4 M and then decreased as the external NaCl increased further. The high intracellular K+ was balanced by the negative charges of the organic osmolytes. At the higher external salt concentrations, it is suggested that Na+ and possibly Cl- ions are internalized to provide osmotic balance. A striking difference of strain Marburg from strain delta H was that yeast extract facilitated growth in high-NaCl-containing medium. The yeast extract supplied only trace NMR-detectable solutes (e.g., betaine) but had a large effect on endogenous glutamate levels, which were significantly decreased. Exogenous choline and glycine, instead of yeast extract, also aided growth in NaCl-containing media. Both solutes were internalized with the choline converted to betaine; the contribution to osmotic balance of these species was 20 to 25% of the total small-molecule pool. These results indicate that M. thermoautotrophicum shows little changes in its internal solutes over a wide range of external NaCl. Furthermore, they illustrate the considerable differences in physiology in the delta H and Marburg strains of this organism." @default.
• W1874277632 created "2016-06-24" @default.
• W1874277632 creator A5013579156 @default.
• W1874277632 creator A5018718060 @default.
• W1874277632 creator A5030214247 @default.
• W1874277632 creator A5038909175 @default.
• W1874277632 creator A5045886913 @default.
• W1874277632 creator A5047196423 @default.
• W1874277632 creator A5071153620 @default.
• W1874277632 date "1994-06-01" @default.
• W1874277632 modified "2023-09-26" @default.
• W1874277632 title "Halotolerance of Methanobacterium thermoautotrophicum delta H and Marburg" @default.
• W1874277632 cites W1496944465 @default.
• W1874277632 cites W1499848412 @default.
• W1874277632 cites W1526900250 @default.
• W1874277632 cites W1569080553 @default.
• W1874277632 cites W1583325045 @default.
• W1874277632 cites W1587663357 @default.
• W1874277632 cites W1594017187 @default.
• W1874277632 cites W1600803307 @default.
• W1874277632 cites W1758862742 @default.
• W1874277632 cites W1819854185 @default.
• W1874277632 cites W1876441925 @default.
• W1874277632 cites W1902268922 @default.
• W1874277632 cites W1908347765 @default.
• W1874277632 cites W1930952597 @default.
• W1874277632 cites W1985342306 @default.
• W1874277632 cites W1992949792 @default.
• W1874277632 cites W1993261695 @default.
• W1874277632 cites W2000541462 @default.
• W1874277632 cites W2014273504 @default.
• W1874277632 cites W2020421681 @default.
• W1874277632 cites W2026704085 @default.
• W1874277632 cites W2029242813 @default.
• W1874277632 cites W2029952963 @default.
• W1874277632 cites W2031669142 @default.
• W1874277632 cites W2078769450 @default.
• W1874277632 cites W2091907883 @default.
• W1874277632 cites W2092533608 @default.
• W1874277632 cites W2106236518 @default.
• W1874277632 cites W2124709562 @default.
• W1874277632 cites W2128635872 @default.
• W1874277632 cites W2137180677 @default.
• W1874277632 cites W2166589843 @default.
• W1874277632 cites W2173654459 @default.
• W1874277632 cites W2394973222 @default.
• W1874277632 cites W2431162702 @default.
• W1874277632 cites W2444216735 @default.
• W1874277632 cites W4211198974 @default.
• W1874277632 cites W4249456417 @default.
• W1874277632 cites W4293247451 @default.
• W1874277632 doi "https://doi.org/10.1128/jb.176.11.3177-3187.1994" @default.
• W1874277632 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/205486" @default.
• W1874277632 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8195071" @default.
• W1874277632 hasPublicationYear "1994" @default.
• W1874277632 type Work @default.
• W1874277632 sameAs 1874277632 @default.
• W1874277632 citedByCount "42" @default.
• W1874277632 countsByYear W18742776322012 @default.
• W1874277632 countsByYear W18742776322013 @default.
• W1874277632 countsByYear W18742776322014 @default.
• W1874277632 countsByYear W18742776322015 @default.
• W1874277632 countsByYear W18742776322017 @default.
• W1874277632 countsByYear W18742776322020 @default.
• W1874277632 countsByYear W18742776322022 @default.
• W1874277632 countsByYear W18742776322023 @default.
• W1874277632 crossrefType "journal-article" @default.
• W1874277632 hasAuthorship W1874277632A5013579156 @default.
• W1874277632 hasAuthorship W1874277632A5018718060 @default.
• W1874277632 hasAuthorship W1874277632A5030214247 @default.
• W1874277632 hasAuthorship W1874277632A5038909175 @default.
• W1874277632 hasAuthorship W1874277632A5045886913 @default.
• W1874277632 hasAuthorship W1874277632A5047196423 @default.
• W1874277632 hasAuthorship W1874277632A5071153620 @default.
• W1874277632 hasBestOaLocation W18742776322 @default.
• W1874277632 hasConcept C104317684 @default.
• W1874277632 hasConcept C105702510 @default.
• W1874277632 hasConcept C12554922 @default.
• W1874277632 hasConcept C127160389 @default.
• W1874277632 hasConcept C129513315 @default.
• W1874277632 hasConcept C129900496 @default.
• W1874277632 hasConcept C134897140 @default.
• W1874277632 hasConcept C13965031 @default.
• W1874277632 hasConcept C168529131 @default.
• W1874277632 hasConcept C178790620 @default.
• W1874277632 hasConcept C185592680 @default.
• W1874277632 hasConcept C18903297 @default.
• W1874277632 hasConcept C2778022156 @default.
• W1874277632 hasConcept C2779824996 @default.
• W1874277632 hasConcept C30992042 @default.
• W1874277632 hasConcept C517785266 @default.
• W1874277632 hasConcept C523546767 @default.
• W1874277632 hasConcept C537181965 @default.
• W1874277632 hasConcept C54355233 @default.
• W1874277632 hasConcept C550995028 @default.
• W1874277632 hasConcept C55493867 @default.
• W1874277632 hasConcept C58415070 @default.
• W1874277632 hasConcept C79879829 @default.

• next