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• W1874330042 abstract "Producing starches with altered composition, structure and novel physico-chemical properties in planta by manipulating the enzymes which are involved in starch metabolism or (over)expressing heterologous enzymes has huge advantages such as broadening the range of starch applications and reducing the costs of the post-harvest starch modification. The starch binding domain (SBD) technology has been extensively explored in our lab for modifying starch in planta and producing so-called “tailored starches”. In this thesis, two different microbial enzymes, E. coli glycogen branching enzyme (GlgB) and Neisseria polysaccharea amylosucrase, were chosen and expressed in potato to modify starch composition or structure by fusing with an SBD. Expression of these different fusion proteins in potato resulted in starch granule morphology and structure alteration severely in different ways. Moreover, expression of SBD-amylosucrase fusion protein in the amylose-containing potato resulted in starch granules with a rough surface, a 2-fold increase in median granule size, and altered physico-chemical properties including higher end viscosity and higher enzymatic digestibility. Furthermore, earlier produced MAT/SBD transformants were analyzed at the transcriptional level using the POCI array. It showed that although expression of MAT in potato resulted in amalgamated starch granules, it did not affect the transcript level of other genes in the starch biosynthetic pathway. Next to these experiments, a tandem CBM25 domain of amylase from Microbacterium aurum was introduced in potato and the results suggested that it can be used as a novel tool for targeting proteins to granules during starch biosynthesis without causing side-effects. All these studies in this thesis demonstrated that the feasibility of modification of starch in planta by expression of heterologous enzymes fused with SBD." @default.
• W1874330042 created "2016-06-24" @default.
• W1874330042 creator A5052315550 @default.
• W1874330042 date "2010-01-01" @default.
• W1874330042 modified "2023-09-25" @default.
• W1874330042 title "Modification of potato starch granule structure and morphology in planta by expression of starch binding domain fusion proteins." @default.
• W1874330042 cites W1470835062 @default.
• W1874330042 cites W1538484906 @default.
• W1874330042 cites W1541963148 @default.
• W1874330042 cites W1596992460 @default.
• W1874330042 cites W1605784734 @default.
• W1874330042 cites W1746255368 @default.
• W1874330042 cites W1909880719 @default.
• W1874330042 cites W1939082967 @default.
• W1874330042 cites W1965240383 @default.
• W1874330042 cites W1968559207 @default.
• W1874330042 cites W1968598729 @default.
• W1874330042 cites W1968630605 @default.
• W1874330042 cites W1970528649 @default.
• W1874330042 cites W1972645466 @default.
• W1874330042 cites W1979682307 @default.
• W1874330042 cites W1980234998 @default.
• W1874330042 cites W1980447828 @default.
• W1874330042 cites W1980749610 @default.
• W1874330042 cites W1980791076 @default.
• W1874330042 cites W1980914396 @default.
• W1874330042 cites W1986662234 @default.
• W1874330042 cites W1990049238 @default.
• W1874330042 cites W1990064498 @default.
• W1874330042 cites W1990899460 @default.
• W1874330042 cites W1996646642 @default.
• W1874330042 cites W1997267426 @default.
• W1874330042 cites W1998471162 @default.
• W1874330042 cites W1998824834 @default.
• W1874330042 cites W2006007183 @default.
• W1874330042 cites W2006525373 @default.
• W1874330042 cites W2010164292 @default.
• W1874330042 cites W2010773458 @default.
• W1874330042 cites W2012558979 @default.
• W1874330042 cites W2014604536 @default.
• W1874330042 cites W2014621592 @default.
• W1874330042 cites W2015360732 @default.
• W1874330042 cites W2015874015 @default.
• W1874330042 cites W2017893870 @default.
• W1874330042 cites W2020451948 @default.
• W1874330042 cites W2020804411 @default.
• W1874330042 cites W2024472645 @default.
• W1874330042 cites W2024556021 @default.
• W1874330042 cites W2025395245 @default.
• W1874330042 cites W2025996508 @default.
• W1874330042 cites W2026568535 @default.
• W1874330042 cites W2027644933 @default.
• W1874330042 cites W2029101038 @default.
• W1874330042 cites W2029285374 @default.
• W1874330042 cites W2029288908 @default.
• W1874330042 cites W2031947186 @default.
• W1874330042 cites W2033063956 @default.
• W1874330042 cites W2035346257 @default.
• W1874330042 cites W2043211647 @default.
• W1874330042 cites W2043390341 @default.
• W1874330042 cites W2048204353 @default.
• W1874330042 cites W2049030276 @default.
• W1874330042 cites W2053255966 @default.
• W1874330042 cites W2055498167 @default.
• W1874330042 cites W2062012256 @default.
• W1874330042 cites W2063816989 @default.
• W1874330042 cites W2068796195 @default.
• W1874330042 cites W2075992873 @default.
• W1874330042 cites W2076536092 @default.
• W1874330042 cites W2076953752 @default.
• W1874330042 cites W2077459067 @default.
• W1874330042 cites W2078923894 @default.
• W1874330042 cites W2079439457 @default.
• W1874330042 cites W2083655284 @default.
• W1874330042 cites W2090080358 @default.
• W1874330042 cites W2090209062 @default.
• W1874330042 cites W2090970850 @default.
• W1874330042 cites W2097552589 @default.
• W1874330042 cites W2097919541 @default.
• W1874330042 cites W2099258488 @default.
• W1874330042 cites W2100837269 @default.
• W1874330042 cites W2103241013 @default.
• W1874330042 cites W2105561958 @default.
• W1874330042 cites W2108929776 @default.
• W1874330042 cites W2108976000 @default.
• W1874330042 cites W2109206751 @default.
• W1874330042 cites W2113079070 @default.
• W1874330042 cites W2114874948 @default.
• W1874330042 cites W2116768772 @default.
• W1874330042 cites W2117135989 @default.
• W1874330042 cites W2120383423 @default.
• W1874330042 cites W2121155037 @default.
• W1874330042 cites W2121631499 @default.
• W1874330042 cites W2126903379 @default.
• W1874330042 cites W2127159557 @default.
• W1874330042 cites W2128274700 @default.
• W1874330042 cites W2128864685 @default.
• W1874330042 cites W2130753366 @default.
• W1874330042 cites W2132277268 @default.
• W1874330042 cites W2133512298 @default.

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