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• W187459506 endingPage "3445" @default.
• W187459506 startingPage "3432" @default.
• W187459506 abstract "The sequences of events regulating thrombin generation during tissue factor-initiated clotting in whole blood at 37 degrees C in which the contact pathway was suppressed with corn trypsin inhibitor are studied using quantitative Western blotting of factor V, prothrombin, platelet factor 4, antithrombin III, and fibrinogen. In addition, fibrinopeptide A (FPA), thrombin-antithrombin III (TAT) complex formation, and prothrombin fragment 1.2 (F1.2) were measured via commercially available enzyme-linked immunosorbent assays (ELISAs). In a typical experiment initiated with 40 pmol/L recombinant tissue factor, visual clot time (4.5 minutes), was preceded by significant cleavage of factor V resulting in 65% factor Va heavy-chain generation but only 10% light-chain formation. At this point, 50% of the platelet factor 4 is released, suggesting that half (approximately 700 pmol/L) of the platelet prothrombinase sites available have been generated. At clot time, approximately 15 nmol/L thrombin B-chain is present; however, analyses of FPA release demonstrate that only 15% of the thrombin is acting on fibrinogen. This thrombin is produced by the action of 7 pmol/L prothrombinase. The maximum rate of thrombin production is reached well after clot time and is consistent with the presence of approximately 150 pmol/L prothrombinase (at about 7 minutes). These results suggest that factor Xa is the limiting factor for thrombin generation. After 60 minutes, 75% of the initial prothrombin (1.24 mumol/L) is consumed yielding 400 nmol/ L prethrombin 2 and 360 nmol/l thrombin (B-chain) products. The sum of these values (800 nmol/L) is similar to the (corrected) F1.2 concentration determined by ELISA. The incomplete cleavage of prothrombin indicates both the prothrombinase complex and the formation of prothrombinase are inhibited in the reaction. TAT complex measured by ELISA is almost equivalent to B-chain concentration, but sodium dodecyl sulfate stable thrombin-antithrombin III complexes are not observed until well after clot formation and are never equivalent to ELISA-TAT values. At the point of clot formation, 80% of the fibrinogen is depleted from the fluid phase, whereas only 35% to 45% of the FPA is released, suggesting a significant incorporation of uncleaved fibrinogen into the initial clot formed." @default.
• W187459506 created "2016-06-24" @default.
• W187459506 creator A5020603244 @default.
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• W187459506 date "1996-11-01" @default.
• W187459506 modified "2023-10-18" @default.
• W187459506 title "Blood clotting in minimally altered whole blood" @default.
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• W187459506 cites W1508996756 @default.
• W187459506 cites W1519085965 @default.
• W187459506 cites W1526463044 @default.
• W187459506 cites W1531124972 @default.
• W187459506 cites W1539235241 @default.
• W187459506 cites W1540403899 @default.
• W187459506 cites W1549602366 @default.
• W187459506 cites W1553185560 @default.
• W187459506 cites W1560970554 @default.
• W187459506 cites W1574671132 @default.
• W187459506 cites W1578821085 @default.
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• W187459506 cites W1582902340 @default.
• W187459506 cites W1584149880 @default.
• W187459506 cites W1588254032 @default.
• W187459506 cites W1589420516 @default.
• W187459506 cites W1601861562 @default.
• W187459506 cites W1603919509 @default.
• W187459506 cites W1605579668 @default.
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• W187459506 cites W1908202237 @default.
• W187459506 cites W1961597147 @default.
• W187459506 cites W1966875441 @default.
• W187459506 cites W1978879711 @default.
• W187459506 cites W1991312270 @default.
• W187459506 cites W1992803891 @default.
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• W187459506 cites W2021930884 @default.
• W187459506 cites W2025562266 @default.
• W187459506 cites W2031677121 @default.
• W187459506 cites W2032279957 @default.
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• W187459506 cites W2150612971 @default.
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• W187459506 cites W244306418 @default.
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• W187459506 doi "https://doi.org/10.1182/blood.v88.9.3432.bloodjournal8893432" @default.
• W187459506 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8896408" @default.
• W187459506 hasPublicationYear "1996" @default.
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