Matches in SemOpenAlex for { <https://semopenalex.org/work/W1874602025> ?p ?o ?g. }
Showing items 1 to 63 of
63
with 100 items per page.
- W1874602025 abstract "In order to fragment deoxyribonucleic acid (DNA) in a random manner, a variety of methods involving the mechanical breakage of DNA have been employed. These include sonication, needle shear, nebulisation, point-sink shearing and passage through a French pressure cell. The new sequencing technologies utilise smaller fragments than those traditionally generated by mechanical breakage. As a result a number of higher throughput and more powerful technologies for random shearing of DNA have been developed including focused acoustic shearing (Covaris Inc) and high power sonication devices (e.g. Sonicman and Bioruptor). These are able to efficiently fragment DNA down to 100 bp and options for multiplexed sample processing are available. This article describes each of these approaches in turn giving the theory behind and the utility of each and giving practical tips where appropriate. Key Concepts: Shearing DNA using mechanical breakage produce random fragments. Shearing DNA by utilising enzymatic action generates bias and produces a set of nonrandom fragments. Several genomic analysis procedures, especially sequencing, require the shearing of DNA to a set of random DNA fragments. Heat generated during physical shearing can cause A–T rich regions of DNA to permanently dissociate. Randomly sheared DNA needs ‘repairing’, typically by incubation with Klenow or T4 polymerase in the presence of dNTPs, in order to make their termini available for ligation." @default.
- W1874602025 created "2016-06-24" @default.
- W1874602025 creator A5024868279 @default.
- W1874602025 date "2010-11-15" @default.
- W1874602025 modified "2023-10-18" @default.
- W1874602025 title "<scp>DNA</scp> : Mechanical Breakage" @default.
- W1874602025 cites W114632038 @default.
- W1874602025 cites W1527744735 @default.
- W1874602025 cites W1597284113 @default.
- W1874602025 cites W1864022502 @default.
- W1874602025 cites W1979186179 @default.
- W1874602025 cites W1993286844 @default.
- W1874602025 cites W2039716761 @default.
- W1874602025 cites W2164975907 @default.
- W1874602025 cites W2374895076 @default.
- W1874602025 doi "https://doi.org/10.1002/9780470015902.a0005333.pub2" @default.
- W1874602025 hasPublicationYear "2010" @default.
- W1874602025 type Work @default.
- W1874602025 sameAs 1874602025 @default.
- W1874602025 citedByCount "5" @default.
- W1874602025 countsByYear W18746020252014 @default.
- W1874602025 countsByYear W18746020252017 @default.
- W1874602025 countsByYear W18746020252019 @default.
- W1874602025 countsByYear W18746020252022 @default.
- W1874602025 crossrefType "other" @default.
- W1874602025 hasAuthorship W1874602025A5024868279 @default.
- W1874602025 hasConcept C12554922 @default.
- W1874602025 hasConcept C152279782 @default.
- W1874602025 hasConcept C159985019 @default.
- W1874602025 hasConcept C185592680 @default.
- W1874602025 hasConcept C192562407 @default.
- W1874602025 hasConcept C2779015675 @default.
- W1874602025 hasConcept C552990157 @default.
- W1874602025 hasConcept C55493867 @default.
- W1874602025 hasConcept C70721500 @default.
- W1874602025 hasConcept C86803240 @default.
- W1874602025 hasConceptScore W1874602025C12554922 @default.
- W1874602025 hasConceptScore W1874602025C152279782 @default.
- W1874602025 hasConceptScore W1874602025C159985019 @default.
- W1874602025 hasConceptScore W1874602025C185592680 @default.
- W1874602025 hasConceptScore W1874602025C192562407 @default.
- W1874602025 hasConceptScore W1874602025C2779015675 @default.
- W1874602025 hasConceptScore W1874602025C552990157 @default.
- W1874602025 hasConceptScore W1874602025C55493867 @default.
- W1874602025 hasConceptScore W1874602025C70721500 @default.
- W1874602025 hasConceptScore W1874602025C86803240 @default.
- W1874602025 hasLocation W18746020251 @default.
- W1874602025 hasOpenAccess W1874602025 @default.
- W1874602025 hasPrimaryLocation W18746020251 @default.
- W1874602025 hasRelatedWork W1983148357 @default.
- W1874602025 hasRelatedWork W2254403527 @default.
- W1874602025 hasRelatedWork W2254548756 @default.
- W1874602025 hasRelatedWork W2359304232 @default.
- W1874602025 hasRelatedWork W2913049120 @default.
- W1874602025 hasRelatedWork W2995257732 @default.
- W1874602025 hasRelatedWork W3042665394 @default.
- W1874602025 hasRelatedWork W3046978314 @default.
- W1874602025 hasRelatedWork W4225255175 @default.
- W1874602025 hasRelatedWork W4293233113 @default.
- W1874602025 isParatext "false" @default.
- W1874602025 isRetracted "false" @default.
- W1874602025 magId "1874602025" @default.
- W1874602025 workType "other" @default.