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- W1875792330 abstract "Abstract The rate-limiting step in many solid-phase immunoassays is associated with the slow kinetics of binding of macro-molecular antigen and conjugate to the immobilized phase. We demonstrate that the use of ultrasonic energy to enhance mass transport across liquid/solid interfaces can dramatically accelerate antigen binding to immobilized antibodies. We use an ultrasound-accelerated procedure with an enzyme-channelling test strip containing glucose oxidase and specific antibody to the alpha-subunit of human choriogonadotropin (HCG) co-immobilized onto a cellulose support. A horseradish peroxidase conjugate of monospecific antibody to the beta-subunit of HCG is used in the liquid phase to complete the immune sandwich. Use of ultrasound to accelerate binding and of enzyme channelling to eliminate wash steps result in a simple two-incubation protocol by which 25 int. units of urinary HCG per liter can be detected visually in less than 20 min of assay time." @default.
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- W1875792330 date "1984-09-01" @default.
- W1875792330 modified "2023-09-25" @default.
- W1875792330 title "Ultrasound-accelerated immunoassay, as exemplified by enzyme immunoassay of choriogonadotropin." @default.
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- W1875792330 doi "https://doi.org/10.1093/clinchem/30.9.1446" @default.
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