FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1877655691> ?p ?o ?g. }

• W1877655691 endingPage "756" @default.
• W1877655691 startingPage "745" @default.
• W1877655691 abstract "ABSTRACT A novel domain of epitopes is expressed by a family of high-Mr proteins at the anterior pole of the germ cell nucleus during spermiogenesis, and later by two low-Mr proteins at the anterior and posterior poles of the nucleus during sperm maturation in the epididymis. Initially, monoclonal antibodies (mAbs) PNT-1 (IgG2b) and PNT-2 (IgG2a) bound to antigens present in a cap-like configuration at the apical pole of the spermatid nucleus at step 5 of spermiogenesis. The distribution of epitopes on the nucleus expanded posteriorly until, in testicular sperm they covered the anterior pole down to the distal limits of the subacrosomal perforatorium. By contrast, sperm from the epididymis and vas deferens bound both mAbs in two distinct regions on the nucleus, one on the dorsal margin of the anterior pole, and the other in a ventral zone at the posterior pole. On SDS-PAGE and isoelectric focusing (IEF) immunoblots, both mAbs bound three major proteins with Mr of ∽80 000,77 000 and 75 000 from spermatids and testicular sperm, and proteins of Mr 50 000 and 48 000 in epididymal and vas deferens sperm. Both the high- and low-Mr protein families were recovered in germ cell nuclear/perinuclear matrices. Their mobilities on SDS-PAGE were not altered by exo- or endoglycosidases or by aminoethylation in denaturing conditions. mAb PNT-1 bound to the sperm proteins with a Ka of 3.53 ×1012 M-1 and mAb PNT-2 with a KB of 2.08 ×1012 M-1. From competition binding data, mAbs PNT-1 to -10 appeared to recognize six adjacent or overlapping epitopes on the same proteins. These data suggest the high-Mr proteins, the thecins, present at the anterior pole of haploid germ cells are processed at the onset of sperm maturation to yield two low-Mr proteins that then occupy two distinct domains at the anterior and posterior poles of the nucleus." @default.
• W1877655691 created "2016-06-24" @default.
• W1877655691 creator A5013518952 @default.
• W1877655691 creator A5023854736 @default.
• W1877655691 creator A5077606970 @default.
• W1877655691 date "1990-08-01" @default.
• W1877655691 modified "2023-10-09" @default.
• W1877655691 title "Temporal expression, polar distribution and transition of an epitope domain in the perinuclear theca during mouse spermatogenesis" @default.
• W1877655691 cites W1489292103 @default.
• W1877655691 cites W1542177054 @default.
• W1877655691 cites W1604083601 @default.
• W1877655691 cites W1606423922 @default.
• W1877655691 cites W1618691523 @default.
• W1877655691 cites W1829588908 @default.
• W1877655691 cites W1963796950 @default.
• W1877655691 cites W1967207742 @default.
• W1877655691 cites W1968822422 @default.
• W1877655691 cites W1977806942 @default.
• W1877655691 cites W1985621084 @default.
• W1877655691 cites W1989690233 @default.
• W1877655691 cites W1994231749 @default.
• W1877655691 cites W2007163501 @default.
• W1877655691 cites W2009889818 @default.
• W1877655691 cites W2023174889 @default.
• W1877655691 cites W2025792687 @default.
• W1877655691 cites W2026371990 @default.
• W1877655691 cites W2034211718 @default.
• W1877655691 cites W2035090247 @default.
• W1877655691 cites W2041560479 @default.
• W1877655691 cites W2042537337 @default.
• W1877655691 cites W2044142379 @default.
• W1877655691 cites W2045255714 @default.
• W1877655691 cites W2046275054 @default.
• W1877655691 cites W2051293423 @default.
• W1877655691 cites W2051627482 @default.
• W1877655691 cites W2057290809 @default.
• W1877655691 cites W2061554547 @default.
• W1877655691 cites W2064069255 @default.
• W1877655691 cites W2071709406 @default.
• W1877655691 cites W2074080513 @default.
• W1877655691 cites W2083453910 @default.
• W1877655691 cites W2086068049 @default.
• W1877655691 cites W2086560719 @default.
• W1877655691 cites W2100837269 @default.
• W1877655691 cites W2101108802 @default.
• W1877655691 cites W2104144885 @default.
• W1877655691 cites W2105867749 @default.
• W1877655691 cites W2107771511 @default.
• W1877655691 cites W2110007409 @default.
• W1877655691 cites W2119460480 @default.
• W1877655691 cites W2128269277 @default.
• W1877655691 cites W2144148386 @default.
• W1877655691 cites W2166518804 @default.
• W1877655691 cites W2181741687 @default.
• W1877655691 cites W4232534952 @default.
• W1877655691 doi "https://doi.org/10.1242/jcs.96.4.745" @default.
• W1877655691 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1704378" @default.
• W1877655691 hasPublicationYear "1990" @default.
• W1877655691 type Work @default.
• W1877655691 sameAs 1877655691 @default.
• W1877655691 citedByCount "30" @default.
• W1877655691 countsByYear W18776556912015 @default.
• W1877655691 crossrefType "journal-article" @default.
• W1877655691 hasAuthorship W1877655691A5013518952 @default.
• W1877655691 hasAuthorship W1877655691A5023854736 @default.
• W1877655691 hasAuthorship W1877655691A5077606970 @default.
• W1877655691 hasConcept C104317684 @default.
• W1877655691 hasConcept C105702510 @default.
• W1877655691 hasConcept C123765429 @default.
• W1877655691 hasConcept C134018914 @default.
• W1877655691 hasConcept C147483822 @default.
• W1877655691 hasConcept C153911025 @default.
• W1877655691 hasConcept C159654299 @default.
• W1877655691 hasConcept C195616568 @default.
• W1877655691 hasConcept C2777394357 @default.
• W1877655691 hasConcept C2778210472 @default.
• W1877655691 hasConcept C2778252442 @default.
• W1877655691 hasConcept C2778619154 @default.
• W1877655691 hasConcept C2779122487 @default.
• W1877655691 hasConcept C2780723820 @default.
• W1877655691 hasConcept C2781087480 @default.
• W1877655691 hasConcept C2781254305 @default.
• W1877655691 hasConcept C542903549 @default.
• W1877655691 hasConcept C54355233 @default.
• W1877655691 hasConcept C86803240 @default.
• W1877655691 hasConcept C95444343 @default.
• W1877655691 hasConceptScore W1877655691C104317684 @default.
• W1877655691 hasConceptScore W1877655691C105702510 @default.
• W1877655691 hasConceptScore W1877655691C123765429 @default.
• W1877655691 hasConceptScore W1877655691C134018914 @default.
• W1877655691 hasConceptScore W1877655691C147483822 @default.
• W1877655691 hasConceptScore W1877655691C153911025 @default.
• W1877655691 hasConceptScore W1877655691C159654299 @default.
• W1877655691 hasConceptScore W1877655691C195616568 @default.
• W1877655691 hasConceptScore W1877655691C2777394357 @default.
• W1877655691 hasConceptScore W1877655691C2778210472 @default.
• W1877655691 hasConceptScore W1877655691C2778252442 @default.
• W1877655691 hasConceptScore W1877655691C2778619154 @default.

• next