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- W1877773044 abstract "Cell wall and cytoplasmic fractions were isolated from mechanically disrupted yeast-phase cells of Blastomyces dermatitidis in an effort to obtain a reliable skin-test antigen. The biological activities of these fractions were compared with those of two blastomycin preparations. The cytoplasmic antigens exhibited a low index of specificity yet exceeded the specificities of the blastomycins. The skin test-active component(s) of the cytoplasmic material had a molecular weight between 10,000 and 30,000 and could easily be concentrated by ultrafiltration on a PM-10 membrane. Unlike the cytoplasmic antigens and blastomycins, an alkali-soluble, water-soluble cell wall antigen effectively distinguished guinea pigs that were sensitized to B. dermatitidis from those sensitized to Histoplasma capsulatum. The biological activity of the yeast-phase antigen could be quantitated, on a weight basis, after purification by ultrafiltration (PM-10 membrane)." @default.
- W1877773044 created "2016-06-24" @default.
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- W1877773044 date "1974-07-01" @default.
- W1877773044 modified "2023-09-27" @default.
- W1877773044 title "Isolation of Skin Test-Active Preparations from Yeast-Phase Cells of Blastomyces dermatitidis" @default.
- W1877773044 cites W1577196389 @default.
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- W1877773044 doi "https://doi.org/10.1128/iai.10.1.42-47.1974" @default.
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