FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1877903228> ?p ?o ?g. }

• W1877903228 endingPage "21" @default.
• W1877903228 startingPage "21" @default.
• W1877903228 abstract "Pseudomonas aeruginosa, an increasingly prevalent opportunistic pathogen, utilizes a type III secretion system for injection of toxins into host cells in order to initiate infection. A crucial component of this system is PcrV, which is essential for cytotoxicity and is found both within the bacterial cytoplasm and localized extracellularly, suggesting that it may play more than one role in Pseudomonas infectivity. LcrV, the homolog of PcrV in Yersinia, has been proposed to participate in effector secretion regulation by interacting with LcrG, which may act as a secretion blocker. Although PcrV also recognizes PcrG within the bacterial cytoplasm, the roles played by the two proteins in type III secretion in Pseudomonas may be different from the ones suggested for their Yersinia counterparts.In this work, we demonstrate by native mass spectrometry that PcrV and PcrG expressed and purified from E. coli form a 1:1 complex in vitro. Circular dichroism results indicate that PcrG is highly unstable in the absence of PcrV; in contrast, both PcrV alone and the PcrV:PcrG complex have high structural integrity. Surface plasmon resonance measurements show that PcrV interacts with PcrG with nanomolar affinity (15.6 nM) and rapid kinetics, an observation which is valid both for the full-length form of PcrG (residues 1-98) as well as a form which lacks the C-terminal 24 residues, which are predicted to have low secondary structure content.PcrV is a crucial component of the type III secretion system of Pseudomonas, but the way in which it participates in toxin secretion is not understood. Here we have characterized the interaction between PcrV and PcrG in vitro, and shown that PcrG is highly unstable. However, it associates readily with PcrV through a region located within its first 74 amino acids to form a high affinity complex. The fact that PcrV associates and dissociates quickly from an unstable molecule points to the transient nature of a PcrV:PcrG complex. These results are in agreement with analyses from pcrV deletion mutants which suggest that PcrV:PcrG may play a different role in effector secretion than the one described for the LcrV:LcrG complex in Yersinia." @default.
• W1877903228 created "2016-06-24" @default.
• W1877903228 creator A5005841631 @default.
• W1877903228 creator A5017617973 @default.
• W1877903228 creator A5046675459 @default.
• W1877903228 creator A5055191235 @default.
• W1877903228 creator A5055611337 @default.
• W1877903228 creator A5057040957 @default.
• W1877903228 creator A5074792224 @default.
• W1877903228 creator A5080267419 @default.
• W1877903228 date "2003-01-01" @default.
• W1877903228 modified "2023-10-18" @default.
• W1877903228 cites W12119413 @default.
• W1877903228 cites W1511786918 @default.
• W1877903228 cites W1821732051 @default.
• W1877903228 cites W1824700284 @default.
• W1877903228 cites W1866781827 @default.
• W1877903228 cites W1926393524 @default.
• W1877903228 cites W1970356669 @default.
• W1877903228 cites W1975511017 @default.
• W1877903228 cites W1990299056 @default.
• W1877903228 cites W2017728221 @default.
• W1877903228 cites W2056085992 @default.
• W1877903228 cites W2056186055 @default.
• W1877903228 cites W2058179337 @default.
• W1877903228 cites W2083861520 @default.
• W1877903228 cites W2096205447 @default.
• W1877903228 cites W2099804686 @default.
• W1877903228 cites W2106811814 @default.
• W1877903228 cites W2122351989 @default.
• W1877903228 cites W2123112413 @default.
• W1877903228 cites W2144878722 @default.
• W1877903228 cites W2150981003 @default.
• W1877903228 cites W2154971985 @default.
• W1877903228 cites W2157521154 @default.
• W1877903228 cites W2158102238 @default.
• W1877903228 cites W2158244329 @default.
• W1877903228 doi "https://doi.org/10.1186/1471-2180-3-21" @default.
• W1877903228 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/270082" @default.
• W1877903228 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/14565848" @default.
• W1877903228 hasPublicationYear "2003" @default.
• W1877903228 type Work @default.
• W1877903228 sameAs 1877903228 @default.
• W1877903228 citedByCount "35" @default.
• W1877903228 countsByYear W18779032282012 @default.
• W1877903228 countsByYear W18779032282014 @default.
• W1877903228 countsByYear W18779032282015 @default.
• W1877903228 countsByYear W18779032282016 @default.
• W1877903228 countsByYear W18779032282017 @default.
• W1877903228 countsByYear W18779032282018 @default.
• W1877903228 countsByYear W18779032282020 @default.
• W1877903228 countsByYear W18779032282021 @default.
• W1877903228 crossrefType "journal-article" @default.
• W1877903228 hasAuthorship W1877903228A5005841631 @default.
• W1877903228 hasAuthorship W1877903228A5017617973 @default.
• W1877903228 hasAuthorship W1877903228A5046675459 @default.
• W1877903228 hasAuthorship W1877903228A5055191235 @default.
• W1877903228 hasAuthorship W1877903228A5055611337 @default.
• W1877903228 hasAuthorship W1877903228A5057040957 @default.
• W1877903228 hasAuthorship W1877903228A5074792224 @default.
• W1877903228 hasAuthorship W1877903228A5080267419 @default.
• W1877903228 hasBestOaLocation W18779032281 @default.
• W1877903228 hasConcept C12554922 @default.
• W1877903228 hasConcept C133571119 @default.
• W1877903228 hasConcept C190062978 @default.
• W1877903228 hasConcept C2776255393 @default.
• W1877903228 hasConcept C2777637488 @default.
• W1877903228 hasConcept C2781463711 @default.
• W1877903228 hasConcept C49039625 @default.
• W1877903228 hasConcept C51785407 @default.
• W1877903228 hasConcept C523546767 @default.
• W1877903228 hasConcept C54355233 @default.
• W1877903228 hasConcept C55493867 @default.
• W1877903228 hasConcept C86803240 @default.
• W1877903228 hasConcept C89423630 @default.
• W1877903228 hasConcept C95444343 @default.
• W1877903228 hasConceptScore W1877903228C12554922 @default.
• W1877903228 hasConceptScore W1877903228C133571119 @default.
• W1877903228 hasConceptScore W1877903228C190062978 @default.
• W1877903228 hasConceptScore W1877903228C2776255393 @default.
• W1877903228 hasConceptScore W1877903228C2777637488 @default.
• W1877903228 hasConceptScore W1877903228C2781463711 @default.
• W1877903228 hasConceptScore W1877903228C49039625 @default.
• W1877903228 hasConceptScore W1877903228C51785407 @default.
• W1877903228 hasConceptScore W1877903228C523546767 @default.
• W1877903228 hasConceptScore W1877903228C54355233 @default.
• W1877903228 hasConceptScore W1877903228C55493867 @default.
• W1877903228 hasConceptScore W1877903228C86803240 @default.
• W1877903228 hasConceptScore W1877903228C89423630 @default.
• W1877903228 hasConceptScore W1877903228C95444343 @default.
• W1877903228 hasIssue "1" @default.
• W1877903228 hasLocation W18779032281 @default.
• W1877903228 hasLocation W18779032282 @default.
• W1877903228 hasLocation W18779032283 @default.
• W1877903228 hasLocation W18779032284 @default.
• W1877903228 hasOpenAccess W1877903228 @default.
• W1877903228 hasPrimaryLocation W18779032281 @default.
• W1877903228 hasRelatedWork W1495119128 @default.

• next