Matches in SemOpenAlex for { <https://semopenalex.org/work/W18781298> ?p ?o ?g. }
Showing items 1 to 65 of
65
with 100 items per page.
- W18781298 abstract "The human carcinoma cell line RPMI 2650 produces autocrinefactors. These are detected by the ability of RPMI 2650conditioned medium (CM) to stimulate growth in soft agar ofRPMI 2650 cells plated at low density The autocrine activityin crude CM can be fractionated by ultrafiltration into alower molecular weight (MW) fraction (Rl-30), whichconcentrates molecules in the X,000-30,000 kDa range, and ahigher MW fraction (R30) with molecules greater than 30,000kDa in a more concentrated form Rl-30 is labile to acid, base and heat treatment, whereas R30 is stable to (and sometimes activated by) these treatments. Boiling of R30, however, renders it labile to acid-, base- and trypsin-treatments CM can be separated into a weakly heparin-bindmg fraction (with stability properties similar to Rl-30), and a non-heparin binding fraction (with stability properties similar to R30) RPMI 2650 cells secrete TGF-a- and TGF-B-hke molecules, but the Rl-30 can be distinguished from these TGFs, and from most other known growth factors, by the unusual combination of acid lability and weak affinity for heparin Since the R30/non-heparin binding fraction is rendered labile by boiling or acid treatment, it may represent a bound or conformâtlonally stable form of a growth factor Growth factor production from RPMI 2650 cells was successfully scaled up to a 2 litre scale." @default.
- W18781298 created "2016-06-24" @default.
- W18781298 creator A5076191726 @default.
- W18781298 date "1992-01-01" @default.
- W18781298 modified "2023-09-26" @default.
- W18781298 title "Production of growth factors from the human carcinoma cell line, RPMI 2650" @default.
- W18781298 hasPublicationYear "1992" @default.
- W18781298 type Work @default.
- W18781298 sameAs 18781298 @default.
- W18781298 citedByCount "1" @default.
- W18781298 crossrefType "dissertation" @default.
- W18781298 hasAuthorship W18781298A5076191726 @default.
- W18781298 hasConcept C128240485 @default.
- W18781298 hasConcept C149629883 @default.
- W18781298 hasConcept C170493617 @default.
- W18781298 hasConcept C185592680 @default.
- W18781298 hasConcept C202751555 @default.
- W18781298 hasConcept C2775960820 @default.
- W18781298 hasConcept C2777557582 @default.
- W18781298 hasConcept C43617362 @default.
- W18781298 hasConcept C50817676 @default.
- W18781298 hasConcept C54355233 @default.
- W18781298 hasConcept C55493867 @default.
- W18781298 hasConcept C81885089 @default.
- W18781298 hasConcept C86803240 @default.
- W18781298 hasConceptScore W18781298C128240485 @default.
- W18781298 hasConceptScore W18781298C149629883 @default.
- W18781298 hasConceptScore W18781298C170493617 @default.
- W18781298 hasConceptScore W18781298C185592680 @default.
- W18781298 hasConceptScore W18781298C202751555 @default.
- W18781298 hasConceptScore W18781298C2775960820 @default.
- W18781298 hasConceptScore W18781298C2777557582 @default.
- W18781298 hasConceptScore W18781298C43617362 @default.
- W18781298 hasConceptScore W18781298C50817676 @default.
- W18781298 hasConceptScore W18781298C54355233 @default.
- W18781298 hasConceptScore W18781298C55493867 @default.
- W18781298 hasConceptScore W18781298C81885089 @default.
- W18781298 hasConceptScore W18781298C86803240 @default.
- W18781298 hasLocation W187812981 @default.
- W18781298 hasOpenAccess W18781298 @default.
- W18781298 hasPrimaryLocation W187812981 @default.
- W18781298 hasRelatedWork W153493228 @default.
- W18781298 hasRelatedWork W1967161065 @default.
- W18781298 hasRelatedWork W1984675553 @default.
- W18781298 hasRelatedWork W1999909339 @default.
- W18781298 hasRelatedWork W2020997182 @default.
- W18781298 hasRelatedWork W2021820069 @default.
- W18781298 hasRelatedWork W2029288548 @default.
- W18781298 hasRelatedWork W2051853085 @default.
- W18781298 hasRelatedWork W2076077117 @default.
- W18781298 hasRelatedWork W2079407183 @default.
- W18781298 hasRelatedWork W2087129197 @default.
- W18781298 hasRelatedWork W2169689879 @default.
- W18781298 hasRelatedWork W2414735653 @default.
- W18781298 hasRelatedWork W2423110038 @default.
- W18781298 hasRelatedWork W2425313322 @default.
- W18781298 hasRelatedWork W1499747003 @default.
- W18781298 hasRelatedWork W2758018492 @default.
- W18781298 hasRelatedWork W2876957757 @default.
- W18781298 hasRelatedWork W2940145565 @default.
- W18781298 hasRelatedWork W3106774049 @default.
- W18781298 isParatext "false" @default.
- W18781298 isRetracted "false" @default.
- W18781298 magId "18781298" @default.
- W18781298 workType "dissertation" @default.