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- W1878559917 abstract "Publisher Summary This chapter illustrates fluorometric titration of urinary riboflavin with an apoflavoprotein. Riboflavin, the flavin moiety of the flavodoxin in egg white, has a distinct fluorescence which is quenched when it combines with an equal molar quantity of the egg white apoprotein. This quenching of the flavin fluorescence makes possible direct titration of urinary riboflavin with the purified apoprotein. The concentration of urinary riboflavin is calculated from the difference in fluorescence before and after the apoprotein titration. The considered assay is a rapid, sensitive, and inexpensive measurement of urinary riboflavin. Several dilutions of a sample including duplicates can be prepared and titrated within minutes because the binding of the riboflavin to the apoprotein is instantaneous. Riboflavin losses are minimized because pretreatment of the sample, except for dilution, is not necessary. The riboflavin concentration of 350–400 samples can be measured with the apoprotein isolated and purified from a single egg. The measurement of riboflavin by the apoprotein titration is dependent on the difference of the fluorescence of the sample before and after the titration." @default.
- W1878559917 created "2016-06-24" @default.
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- W1878559917 date "1986-01-01" @default.
- W1878559917 modified "2023-09-26" @default.
- W1878559917 title "[37] Fluorometric titration of urinary riboflavin with an apoflavoprotein" @default.
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- W1878559917 doi "https://doi.org/10.1016/0076-6879(86)22175-8" @default.
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