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- W1878562488 abstract "The freeze substitution histological tech nique allows the indirect light microscopicobser vation and the quantitative evaluation of ice crystal size in frozen tissues. Theuseofchemical fixatives in substitu ting fluids improves morpholo gical and histochemical preservation of the tissue. Fixation conditions become important since this step can introduce modifications in crystal sizes . Effects of temperature on: a) diffusion rate of fixing solution in tissue, b) recrystallization rate of ice in frozen beef and c) variations of the froze~ water fraction were ana l yzed, establishing that 1sothermal freeze fixatio n constitutes an appropriate method for histological observation. Fixing at temperatures lower than that of the sample, in an attempt to reduce recrystallization effects , involves an increase of the frozen water f~action i.n the tissue leading to modifications in s12e of 1ce crystals and to changes in the" @default.
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- W1878562488 date "1986-01-01" @default.
- W1878562488 modified "2023-09-28" @default.
- W1878562488 title "Fixing conditions in the freeze substitution technique for light microscopy observation of frozen beef tissue" @default.
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