Matches in SemOpenAlex for { <https://semopenalex.org/work/W1878647250> ?p ?o ?g. }
- W1878647250 endingPage "1578" @default.
- W1878647250 startingPage "1568" @default.
- W1878647250 abstract "In bacterial cells, processing of double-stranded DNA breaks for repair by homologous recombination is dependent upon the recombination hotspot sequence Chi and is catalysed by either an AddAB- or RecBCD-type helicase-nuclease. Here, we report the crystal structure of AddAB bound to DNA. The structure allows identification of a putative Chi-recognition site in an inactivated helicase domain of the AddB subunit. By generating mutant protein complexes that do not respond to Chi, we show that residues responsible for Chi recognition are located in positions equivalent to the signature motifs of a conventional helicase. Comparison with the related RecBCD complex, which recognizes a different Chi sequence, provides further insight into the structural basis for sequence-specific ssDNA recognition. The structure suggests a simple mechanism for DNA break processing, explains how AddAB and RecBCD can accomplish the same overall reaction with different sets of functional modules and reveals details of the role of an Fe-S cluster in protein stability and DNA binding." @default.
- W1878647250 created "2016-06-24" @default.
- W1878647250 creator A5035406639 @default.
- W1878647250 creator A5044522250 @default.
- W1878647250 creator A5052181591 @default.
- W1878647250 creator A5055389331 @default.
- W1878647250 creator A5065892644 @default.
- W1878647250 creator A5089523004 @default.
- W1878647250 date "2012-02-03" @default.
- W1878647250 modified "2023-10-07" @default.
- W1878647250 title "Insights into Chi recognition from the structure of an AddAB-type helicase-nuclease complex" @default.
- W1878647250 cites W1799442365 @default.
- W1878647250 cites W1953968639 @default.
- W1878647250 cites W1965531809 @default.
- W1878647250 cites W1967667819 @default.
- W1878647250 cites W1978846042 @default.
- W1878647250 cites W1979208058 @default.
- W1878647250 cites W1979243502 @default.
- W1878647250 cites W1983732912 @default.
- W1878647250 cites W1991988366 @default.
- W1878647250 cites W1996984611 @default.
- W1878647250 cites W2000840258 @default.
- W1878647250 cites W2000988465 @default.
- W1878647250 cites W2001641653 @default.
- W1878647250 cites W2005402849 @default.
- W1878647250 cites W2011700794 @default.
- W1878647250 cites W2012095700 @default.
- W1878647250 cites W2013083986 @default.
- W1878647250 cites W2017421343 @default.
- W1878647250 cites W2026052721 @default.
- W1878647250 cites W2026133647 @default.
- W1878647250 cites W2030798502 @default.
- W1878647250 cites W2035503835 @default.
- W1878647250 cites W2039181331 @default.
- W1878647250 cites W2044443934 @default.
- W1878647250 cites W2046276235 @default.
- W1878647250 cites W2050320436 @default.
- W1878647250 cites W2051922054 @default.
- W1878647250 cites W2064402458 @default.
- W1878647250 cites W2064853448 @default.
- W1878647250 cites W2065679107 @default.
- W1878647250 cites W2067342093 @default.
- W1878647250 cites W2080380418 @default.
- W1878647250 cites W2080709317 @default.
- W1878647250 cites W2090953848 @default.
- W1878647250 cites W2094888311 @default.
- W1878647250 cites W2099418917 @default.
- W1878647250 cites W2100929591 @default.
- W1878647250 cites W2105439835 @default.
- W1878647250 cites W2106226576 @default.
- W1878647250 cites W2107901234 @default.
- W1878647250 cites W2110893691 @default.
- W1878647250 cites W2110984313 @default.
- W1878647250 cites W2114382972 @default.
- W1878647250 cites W2117602431 @default.
- W1878647250 cites W2117791924 @default.
- W1878647250 cites W2122339645 @default.
- W1878647250 cites W2122571070 @default.
- W1878647250 cites W2123026180 @default.
- W1878647250 cites W2123965484 @default.
- W1878647250 cites W2128298547 @default.
- W1878647250 cites W2133279612 @default.
- W1878647250 cites W2140244045 @default.
- W1878647250 cites W2140886363 @default.
- W1878647250 cites W2144081223 @default.
- W1878647250 cites W2145532900 @default.
- W1878647250 cites W2146994204 @default.
- W1878647250 cites W2151541959 @default.
- W1878647250 cites W2153556669 @default.
- W1878647250 cites W2154772302 @default.
- W1878647250 cites W2158266834 @default.
- W1878647250 cites W2158602985 @default.
- W1878647250 cites W2159431285 @default.
- W1878647250 cites W2162800127 @default.
- W1878647250 cites W2164837183 @default.
- W1878647250 cites W2169953870 @default.
- W1878647250 cites W2180229411 @default.
- W1878647250 doi "https://doi.org/10.1038/emboj.2012.9" @default.
- W1878647250 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3321194" @default.
- W1878647250 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22307084" @default.
- W1878647250 hasPublicationYear "2012" @default.
- W1878647250 type Work @default.
- W1878647250 sameAs 1878647250 @default.
- W1878647250 citedByCount "56" @default.
- W1878647250 countsByYear W18786472502012 @default.
- W1878647250 countsByYear W18786472502013 @default.
- W1878647250 countsByYear W18786472502014 @default.
- W1878647250 countsByYear W18786472502015 @default.
- W1878647250 countsByYear W18786472502016 @default.
- W1878647250 countsByYear W18786472502017 @default.
- W1878647250 countsByYear W18786472502018 @default.
- W1878647250 countsByYear W18786472502019 @default.
- W1878647250 countsByYear W18786472502020 @default.
- W1878647250 countsByYear W18786472502021 @default.
- W1878647250 countsByYear W18786472502022 @default.
- W1878647250 crossrefType "journal-article" @default.
- W1878647250 hasAuthorship W1878647250A5035406639 @default.
- W1878647250 hasAuthorship W1878647250A5044522250 @default.