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- W1880265280 abstract "Corynebacterium ulcerans is an emerging pathogen transmitted by a zoonotic pathway to humans. Despite rising numbers of infections and potentially fatal outcomes, data on the colonization of the human host are lacking up to now. In this study, adhesion of two C. ulcerans isolates to human epithelial cells, invasion of host cells and the function of two putative virulence factors with respect to these processes were investigated. C. ulcerans strains BR-AD22 and 809 were able to adhere to Detroit562 and HeLa cells, and invade these epithelial cell lines with a rate comparable to other pathogens as shown by scanning electron microscopy, fluorescence microscopy and replication assays. Infection led to detrimental effects on the cells as deduced from measurements of transepithelial resistance. Mutant strains of putative virulence factors phospholipase D and DIP0733 homologue CULC22_00609 generated in this study showed no influence on colonization under the experimental conditions tested. The data presented here indicate a high infectious potential of this emerging pathogen." @default.
- W1880265280 created "2016-06-24" @default.
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- W1880265280 date "2015-08-01" @default.
- W1880265280 modified "2023-09-27" @default.
- W1880265280 title "Colonization of human epithelial cell lines by Corynebacterium ulcerans from human and animal sources" @default.
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- W1880265280 doi "https://doi.org/10.1099/mic.0.000121" @default.
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