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- W188086965 abstract "This chapter describes the assay of three enzymatic activities, each serving as an example for one of the three major approaches utilized in testing enzymatic activities in mollicutes: the spectrophotometric, radioisotopic, and the chromatographic approach. There are about 150–175 cytoplasmic enzymatic activities recognized in Mollicutes. These activities are associated with the glycolysis and pentose phosphate pathways and routes for the synthesis or interconversion of aromatic amino acids, lipids, purines, pyrimidines, and nucleic acids. The three activities are PP i -dependent phosphofructokinase, deoxyuridine triphosphatase (dUTPase), and pyrophosphate-dependent deoxyguanosine kinase. These particular enzymes were chosen because the analysis of each one emphasizes a different major biochemical technique frequently employed in the study of mollicute metabolism. These particular assays are consequential in a metabolic classification of the mollicutes that parallels a phylogeny based on 16S recombinant RNA sequences. DUTPase is found in all cells in nature except for some mollicutes. In free-living organisms, dUTPase is located in the cytoplasm." @default.
- W188086965 created "2016-06-24" @default.
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- W188086965 date "1995-01-01" @default.
- W188086965 modified "2023-09-25" @default.
- W188086965 title "METHODS FOR TESTING METABOLIC ACTIVITIES IN MOLLICUTES" @default.
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- W188086965 doi "https://doi.org/10.1016/b978-012583805-4/50029-7" @default.
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