FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1881516094> ?p ?o ?g. }

• W1881516094 endingPage "e0140900" @default.
• W1881516094 startingPage "e0140900" @default.
• W1881516094 abstract "The Hepatitis C virus (HCV) infection exhibits a high global prevalence frequently associated with hepatocellular carcinoma, taking years to develop. Despite the standardization of highly sensitive HCV quantitative RT-PCR (qRT-PCR) detection methods, false-negative diagnoses may be generated with current methods, mainly due to the presence of PCR inhibitors and/or low viral loads in the patient's sample. These false-negative diagnoses impact both public health systems, in developing countries, and an in lesser extent, in developed countries, including both the risk of virus transmission during organ transplantation and/or blood transfusion and the quality of the antiviral treatment monitoring. To adopt an appropriate therapeutic strategy to improve the patient's prognosis, it is urgent to increase the HCV detection sensitivity. Based upon previous studies on HBV, we worked on the capacity of the scavenger acute phase protein, Apolipoprotein H (ApoH) to interact with HCV. Using different approaches, including immunoassays, antibody-inhibition, oxidation, ultracentrifugation, electron microscopy and RT-PCR analyses, we demonstrated specific interactions between HCV particles and ApoH. Moreover, when using a two-step HCV detection process, including capture of HCV by ApoH-coated nanomagnetic beads and a home-made real-time HCV-RT-PCR, we confirmed the presence of HCV for all samples from a clinical collection of HCV-seropositive patients exhibiting an RT-PCR COBAS® TaqMan® HCV Test, v2.0 (COBAS)-positive result. In contrast, for HCV-seropositive patients with either low HCV-load as determined with COBAS or exhibiting HCV-negative COBAS results, the addition of the two-step ApoH-HCV-capture and HCV-detection process was able to increase the sensitivity of HCV detection or more interestingly, detect in a genotype sequence-independent manner, a high-proportion (44%) of HCV/RNA-positive among the COBAS HCV-negative patients. Thus, the immune interaction between ApoH and HCV could be used as a sample preparation tool to enrich and/or cleanse HCV patient's samples to enhance the detection sensitivity of HCV and therefore significantly reduce the numbers of false-negative HCV diagnosis results." @default.
• W1881516094 created "2016-06-24" @default.
• W1881516094 creator A5012601539 @default.
• W1881516094 creator A5013555148 @default.
• W1881516094 creator A5014926274 @default.
• W1881516094 creator A5035590367 @default.
• W1881516094 creator A5038741105 @default.
• W1881516094 creator A5056055382 @default.
• W1881516094 creator A5069460981 @default.
• W1881516094 creator A5080295259 @default.
• W1881516094 creator A5080896357 @default.
• W1881516094 creator A5082932097 @default.
• W1881516094 date "2015-10-26" @default.
• W1881516094 modified "2023-10-01" @default.
• W1881516094 title "Interactions between Hepatitis C Virus and the Human Apolipoprotein H Acute Phase Protein: A Tool for a Sensitive Detection of the Virus" @default.
• W1881516094 cites W140271323 @default.
• W1881516094 cites W1497989266 @default.
• W1881516094 cites W1498307929 @default.
• W1881516094 cites W1500801849 @default.
• W1881516094 cites W1537390604 @default.
• W1881516094 cites W1539669821 @default.
• W1881516094 cites W1594429335 @default.
• W1881516094 cites W1966967317 @default.
• W1881516094 cites W1971668677 @default.
• W1881516094 cites W1973165481 @default.
• W1881516094 cites W1973958554 @default.
• W1881516094 cites W1985947768 @default.
• W1881516094 cites W1986505810 @default.
• W1881516094 cites W1988192085 @default.
• W1881516094 cites W1988919271 @default.
• W1881516094 cites W1994441172 @default.
• W1881516094 cites W1999898230 @default.
• W1881516094 cites W2001413253 @default.
• W1881516094 cites W2002040221 @default.
• W1881516094 cites W2003104968 @default.
• W1881516094 cites W2010908721 @default.
• W1881516094 cites W2012386121 @default.
• W1881516094 cites W2014424309 @default.
• W1881516094 cites W2014456395 @default.
• W1881516094 cites W2014742624 @default.
• W1881516094 cites W2015489330 @default.
• W1881516094 cites W2015690313 @default.
• W1881516094 cites W2016065477 @default.
• W1881516094 cites W2021762717 @default.
• W1881516094 cites W2022687771 @default.
• W1881516094 cites W2026543492 @default.
• W1881516094 cites W2028207573 @default.
• W1881516094 cites W2028668919 @default.
• W1881516094 cites W2030675713 @default.
• W1881516094 cites W2034831369 @default.
• W1881516094 cites W2035216554 @default.
• W1881516094 cites W2036306822 @default.
• W1881516094 cites W2049746784 @default.
• W1881516094 cites W2050287837 @default.
• W1881516094 cites W2050515555 @default.
• W1881516094 cites W2051220377 @default.
• W1881516094 cites W2052821631 @default.
• W1881516094 cites W2054862747 @default.
• W1881516094 cites W2058954135 @default.
• W1881516094 cites W2059697503 @default.
• W1881516094 cites W2063244569 @default.
• W1881516094 cites W2064113906 @default.
• W1881516094 cites W2069591287 @default.
• W1881516094 cites W2069597046 @default.
• W1881516094 cites W2076955144 @default.
• W1881516094 cites W2078364031 @default.
• W1881516094 cites W2082205790 @default.
• W1881516094 cites W2083924203 @default.
• W1881516094 cites W2085457459 @default.
• W1881516094 cites W2086356952 @default.
• W1881516094 cites W2087583050 @default.
• W1881516094 cites W2087716056 @default.
• W1881516094 cites W2093870776 @default.
• W1881516094 cites W2105291849 @default.
• W1881516094 cites W2107397862 @default.
• W1881516094 cites W2118320694 @default.
• W1881516094 cites W2123394168 @default.
• W1881516094 cites W2128515651 @default.
• W1881516094 cites W2130525876 @default.
• W1881516094 cites W2131342968 @default.
• W1881516094 cites W2131344889 @default.
• W1881516094 cites W2131668666 @default.
• W1881516094 cites W2132537748 @default.
• W1881516094 cites W2135494094 @default.
• W1881516094 cites W2136896260 @default.
• W1881516094 cites W2143639253 @default.
• W1881516094 cites W2151564108 @default.
• W1881516094 cites W2152112546 @default.
• W1881516094 cites W2153795289 @default.
• W1881516094 cites W2160174559 @default.
• W1881516094 cites W2161336617 @default.
• W1881516094 cites W2164286719 @default.
• W1881516094 cites W2185919342 @default.
• W1881516094 cites W2466462007 @default.
• W1881516094 cites W4302081985 @default.
• W1881516094 doi "https://doi.org/10.1371/journal.pone.0140900" @default.
• W1881516094 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4621047" @default.
• W1881516094 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26502286" @default.

• next