Matches in SemOpenAlex for { <https://semopenalex.org/work/W188226357> ?p ?o ?g. }
Showing items 1 to 77 of
77
with 100 items per page.
- W188226357 endingPage "15" @default.
- W188226357 startingPage "409" @default.
- W188226357 abstract "To sequence the complete genome of CVS-11 strain and establish a reverse genetic system of CVS-11 to further study its pathogenic mechanism, virulence genes and antigenic sites.We amplified12 fragments covering the complete genome of the CVS-11 strain by RT-PCR, and then cloned to pEASY-Blunt vector for sequencing the complete genome of CVS-11. We analyzed single restriction enzyme sites of the full length cDNA of the CVS-11 strain by DNAMAN and designed 4 pairs of specific primers. We amplified the full-length cDNA of CVS-11 by RT-PCR. We obtained four fragments and cloned into pcDNA3. 1. We named the full-length cDNA plasmid pcDNA3. 1-CVS-11. We also cloned helper plasmids pcDNA3.1-N, P, L and G expressing N, P, L and G protein of CVS-11 strain, respectively. We co-transfected NA cells with the full-length plasmid and four helper plasmids. We identified the supernatant of the transfected and then passaged NA cells by immunofluorescence staining and RT-PCR and found the recombinant virus rCVS-11 rescued successfully.Sequencing results showed that the complete genome of CVS-11 was composed of 11 927 nucleotides. The complete genome of CVS-11 encoded 5 structure proteins and gene array was the same as other reported rabies viruses. We successfully constructed a reverse genetic system of CVS-11, namely the full length plasmid pcDNA3. 1-CVS-11 and 4 help plasmids pcDNA3. 1-N, P, L, G and rescued the rCVS-11 from a full-length infectious cDNA clone.The reverse genetic system of the CVS-11 strain laid the foundation for future studies on rabies virus." @default.
- W188226357 created "2016-06-24" @default.
- W188226357 creator A5006028447 @default.
- W188226357 creator A5017651779 @default.
- W188226357 creator A5017985995 @default.
- W188226357 creator A5020084371 @default.
- W188226357 creator A5034517280 @default.
- W188226357 creator A5034746165 @default.
- W188226357 creator A5055631705 @default.
- W188226357 creator A5060507902 @default.
- W188226357 creator A5072861069 @default.
- W188226357 creator A5078677061 @default.
- W188226357 creator A5080098344 @default.
- W188226357 creator A5085325895 @default.
- W188226357 date "2013-04-04" @default.
- W188226357 modified "2023-09-27" @default.
- W188226357 title "[Sequencing the complete genome of rabies virus CVS-11 strain and constructing its full-length infectious cDNA clone]." @default.
- W188226357 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23858717" @default.
- W188226357 hasPublicationYear "2013" @default.
- W188226357 type Work @default.
- W188226357 sameAs 188226357 @default.
- W188226357 citedByCount "1" @default.
- W188226357 countsByYear W1882263572022 @default.
- W188226357 crossrefType "journal-article" @default.
- W188226357 hasAuthorship W188226357A5006028447 @default.
- W188226357 hasAuthorship W188226357A5017651779 @default.
- W188226357 hasAuthorship W188226357A5017985995 @default.
- W188226357 hasAuthorship W188226357A5020084371 @default.
- W188226357 hasAuthorship W188226357A5034517280 @default.
- W188226357 hasAuthorship W188226357A5034746165 @default.
- W188226357 hasAuthorship W188226357A5055631705 @default.
- W188226357 hasAuthorship W188226357A5060507902 @default.
- W188226357 hasAuthorship W188226357A5072861069 @default.
- W188226357 hasAuthorship W188226357A5078677061 @default.
- W188226357 hasAuthorship W188226357A5080098344 @default.
- W188226357 hasAuthorship W188226357A5085325895 @default.
- W188226357 hasConcept C104317684 @default.
- W188226357 hasConcept C141231307 @default.
- W188226357 hasConcept C153911025 @default.
- W188226357 hasConcept C159047783 @default.
- W188226357 hasConcept C187882448 @default.
- W188226357 hasConcept C22744801 @default.
- W188226357 hasConcept C2522874641 @default.
- W188226357 hasConcept C2777961786 @default.
- W188226357 hasConcept C54355233 @default.
- W188226357 hasConcept C86803240 @default.
- W188226357 hasConceptScore W188226357C104317684 @default.
- W188226357 hasConceptScore W188226357C141231307 @default.
- W188226357 hasConceptScore W188226357C153911025 @default.
- W188226357 hasConceptScore W188226357C159047783 @default.
- W188226357 hasConceptScore W188226357C187882448 @default.
- W188226357 hasConceptScore W188226357C22744801 @default.
- W188226357 hasConceptScore W188226357C2522874641 @default.
- W188226357 hasConceptScore W188226357C2777961786 @default.
- W188226357 hasConceptScore W188226357C54355233 @default.
- W188226357 hasConceptScore W188226357C86803240 @default.
- W188226357 hasIssue "4" @default.
- W188226357 hasLocation W1882263571 @default.
- W188226357 hasOpenAccess W188226357 @default.
- W188226357 hasPrimaryLocation W1882263571 @default.
- W188226357 hasRelatedWork W1571771606 @default.
- W188226357 hasRelatedWork W1968976175 @default.
- W188226357 hasRelatedWork W2143709720 @default.
- W188226357 hasRelatedWork W2279576894 @default.
- W188226357 hasRelatedWork W2351912091 @default.
- W188226357 hasRelatedWork W2362096941 @default.
- W188226357 hasRelatedWork W2380100330 @default.
- W188226357 hasRelatedWork W2382744586 @default.
- W188226357 hasRelatedWork W2524850911 @default.
- W188226357 hasRelatedWork W2620737981 @default.
- W188226357 hasVolume "53" @default.
- W188226357 isParatext "false" @default.
- W188226357 isRetracted "false" @default.
- W188226357 magId "188226357" @default.
- W188226357 workType "article" @default.