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• W1884874540 abstract "ABSTRACT Replacement of Escherichia coli ’s RecBCD function with phage λ’s Red function generates a strain whose chromosome recombines with short linear DNA fragments at a greatly elevated rate. The rate is at least 70-fold higher than that exhibited by a recBC sbcBC or recD strain. The value of the system is highlighted by gene replacement with a PCR-generated DNA fragment. The Δ recBCD ::P lac -red kan replacement allele can be P1 transduced to other E. coli strains, making the hyper-Rec phenotype easily transferable." @default.
• W1884874540 created "2016-06-24" @default.
• W1884874540 creator A5039543072 @default.
• W1884874540 date "1998-04-15" @default.
• W1884874540 modified "2023-10-15" @default.
• W1884874540 title "Use of Bacteriophage λ Recombination Functions To Promote Gene Replacement in <i>Escherichia coli</i>" @default.
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• W1884874540 doi "https://doi.org/10.1128/jb.180.8.2063-2071.1998" @default.
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