Matches in SemOpenAlex for { <https://semopenalex.org/work/W1885087135> ?p ?o ?g. }
Showing items 1 to 90 of
90
with 100 items per page.
- W1885087135 abstract "We have constructed a recombinant ( pMOP ) which is based on the bacterial plasmid pML2 and contains bovine papilloma virus type 1 (BPV 1) sequences linked to an artificial mouse dihydrofolate reductase (DHFR) transcription unit. This unit consists of the SV40 early gene promoter, a complete DHFR coding sequence and splice and polyadenylation sites from a rabbit beta-globin gene. Intact pMOP or a fragment thereof devoid of pML2 sequences will transform mouse cells to methotrexate resistance. The lines obtained contain approximately 200 copies of the DHFR transcription unit. In no case, however, did we find evidence of extrachromosomal maintenance of BPV 1 or DHFR sequences. One line, when selected for resistance to elevated levels of methotrexate, contained amplified copies of a 'novel' DHFR transcription unit resulting from fusion of two normal units. This line contained the DHFR RNA species present in the parent line plus some additional species. The structure of these various RNA species was determined and evidence found for the use of cryptic splice and polyadenylation sites." @default.
- W1885087135 created "2016-06-24" @default.
- W1885087135 creator A5013775511 @default.
- W1885087135 date "1984-04-01" @default.
- W1885087135 modified "2023-09-26" @default.
- W1885087135 title "Selective amplification in methotrexate-resistant mouse cells of an artificial dihydrofolate reductase transcription unit making use of cryptic splicing and polyadenylation sites." @default.
- W1885087135 cites W1534119891 @default.
- W1885087135 cites W1853713129 @default.
- W1885087135 cites W1967299411 @default.
- W1885087135 cites W1968647996 @default.
- W1885087135 cites W1971686894 @default.
- W1885087135 cites W19720083 @default.
- W1885087135 cites W1976491544 @default.
- W1885087135 cites W1982799815 @default.
- W1885087135 cites W1984513339 @default.
- W1885087135 cites W1988178079 @default.
- W1885087135 cites W1996890445 @default.
- W1885087135 cites W1998162329 @default.
- W1885087135 cites W2009959065 @default.
- W1885087135 cites W2023557079 @default.
- W1885087135 cites W2030097117 @default.
- W1885087135 cites W2035655668 @default.
- W1885087135 cites W2040276985 @default.
- W1885087135 cites W2049635421 @default.
- W1885087135 cites W2057769791 @default.
- W1885087135 cites W2057944550 @default.
- W1885087135 cites W2063539244 @default.
- W1885087135 cites W2104855527 @default.
- W1885087135 cites W2117477363 @default.
- W1885087135 cites W2762374987 @default.
- W1885087135 cites W87017783 @default.
- W1885087135 doi "https://doi.org/10.1002/j.1460-2075.1984.tb01903.x" @default.
- W1885087135 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/557445" @default.
- W1885087135 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6202514" @default.
- W1885087135 hasPublicationYear "1984" @default.
- W1885087135 type Work @default.
- W1885087135 sameAs 1885087135 @default.
- W1885087135 citedByCount "5" @default.
- W1885087135 crossrefType "journal-article" @default.
- W1885087135 hasAuthorship W1885087135A5013775511 @default.
- W1885087135 hasConcept C104317684 @default.
- W1885087135 hasConcept C138885662 @default.
- W1885087135 hasConcept C142575336 @default.
- W1885087135 hasConcept C153911025 @default.
- W1885087135 hasConcept C179926584 @default.
- W1885087135 hasConcept C2781320022 @default.
- W1885087135 hasConcept C41895202 @default.
- W1885087135 hasConcept C54355233 @default.
- W1885087135 hasConcept C54458228 @default.
- W1885087135 hasConcept C67705224 @default.
- W1885087135 hasConcept C86803240 @default.
- W1885087135 hasConceptScore W1885087135C104317684 @default.
- W1885087135 hasConceptScore W1885087135C138885662 @default.
- W1885087135 hasConceptScore W1885087135C142575336 @default.
- W1885087135 hasConceptScore W1885087135C153911025 @default.
- W1885087135 hasConceptScore W1885087135C179926584 @default.
- W1885087135 hasConceptScore W1885087135C2781320022 @default.
- W1885087135 hasConceptScore W1885087135C41895202 @default.
- W1885087135 hasConceptScore W1885087135C54355233 @default.
- W1885087135 hasConceptScore W1885087135C54458228 @default.
- W1885087135 hasConceptScore W1885087135C67705224 @default.
- W1885087135 hasConceptScore W1885087135C86803240 @default.
- W1885087135 hasLocation W18850871351 @default.
- W1885087135 hasLocation W18850871352 @default.
- W1885087135 hasOpenAccess W1885087135 @default.
- W1885087135 hasPrimaryLocation W18850871351 @default.
- W1885087135 hasRelatedWork W104844502 @default.
- W1885087135 hasRelatedWork W112760532 @default.
- W1885087135 hasRelatedWork W1492794412 @default.
- W1885087135 hasRelatedWork W1548981150 @default.
- W1885087135 hasRelatedWork W1783347833 @default.
- W1885087135 hasRelatedWork W1832674718 @default.
- W1885087135 hasRelatedWork W1856438741 @default.
- W1885087135 hasRelatedWork W1963587929 @default.
- W1885087135 hasRelatedWork W1975642238 @default.
- W1885087135 hasRelatedWork W1978667710 @default.
- W1885087135 hasRelatedWork W1989299721 @default.
- W1885087135 hasRelatedWork W2013689155 @default.
- W1885087135 hasRelatedWork W2027955922 @default.
- W1885087135 hasRelatedWork W2053628284 @default.
- W1885087135 hasRelatedWork W2057401426 @default.
- W1885087135 hasRelatedWork W2069463752 @default.
- W1885087135 hasRelatedWork W2081525900 @default.
- W1885087135 hasRelatedWork W2106569201 @default.
- W1885087135 hasRelatedWork W2114565625 @default.
- W1885087135 hasRelatedWork W2129120165 @default.
- W1885087135 isParatext "false" @default.
- W1885087135 isRetracted "false" @default.
- W1885087135 magId "1885087135" @default.
- W1885087135 workType "article" @default.