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• W1885092012 abstract "The Km value for 6-deoxyglucose influx into Chlorella vulgaris was found to be 0.21 mM and for efflux it was 21 mM. The sugar was accumulated up to 1500-fold, an accumulation which cannot be explained by the difference in Km values. Using these Km values together with estimated relative translocation constants for carrier and carrier-substrate it was possible to determine Ks values. The Ks value thus obtained for the carrier at the inner side of the membrane was 70 times larger than that on the outer side. Although different Km values to the ones given above for influx as well as for efflux were obtained when the measurements were carried out in the presence of high sugar concentrations at the opposite side of the membrane, the calculations yielded almost identical Ks values (Table 3). The experimentally observed accumulation of 6-deoxyglucose can be well explained when the different Ks values and the estimated difference of 25-fold between the translocation constants for outward as compared to inward “movement” of free carrier is considered." @default.
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• W1885092012 date "1973-11-01" @default.
• W1885092012 modified "2023-09-26" @default.
• W1885092012 title "The Active Hexose-Uptake System of Chlorella vulgaris. Km-Values for 6-Deoxyglucose Influx and Efflux and Their Contribution to Sugar Accumulation" @default.
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• W1885092012 doi "https://doi.org/10.1111/j.1432-1033.1973.tb03117.x" @default.
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