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• W1886434626 abstract "In airway smooth muscle (ASM), ACh induces propagating intracellular Ca 2+ concentration ([Ca 2+ ] i ) oscillations (5–30 Hz). We hypothesized that, in ASM, coupling of elevations and reductions in [Ca 2+ ] i to force generation and relaxation (excitation-contraction coupling) is slower than ACh-induced [Ca 2+ ] i oscillations, leading to stable force generation. When we used real-time confocal imaging, the delay between elevated [Ca 2+ ] i and contraction in intact porcine ASM cells was found to be ∼450 ms. In β-escin-permeabilized ASM strips, photolytic release of caged Ca 2+ resulted in force generation after ∼800 ms. When calmodulin (CaM) was added, this delay was shortened to ∼500 ms. In the presence of exogenous CaM and 100 μM Ca 2+ , photolytic release of caged ATP led to force generation after ∼80 ms. These results indicated significant delays due to CaM mobilization and Ca 2+ -CaM activation of myosin light chain kinase but much shorter delays introduced by myosin light chain kinase-induced phosphorylation of the regulatory myosin light chain MLC 20 and cross-bridge recruitment. This was confirmed by prior thiophosphorylation of MLC 20 , in which force generation occurred ∼50 ms after photolytic release of caged ATP, approximating the delay introduced by cross-bridge recruitment alone. The time required to reach maximum steady-state force was >15 s. Rapid chelation of [Ca 2+ ] i after photolytic release of caged diazo-2 resulted in relaxation after a delay of ∼1.2 s and 50% reduction in force after ∼57 s. We conclude that in ASM cells agonist-induced [Ca 2+ ] i oscillations are temporally and spatially integrated during excitation-contraction coupling, resulting in stable force production." @default.
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• W1886434626 date "2001-11-01" @default.
• W1886434626 modified "2023-10-16" @default.
• W1886434626 title "Temporal aspects of excitation-contraction coupling in airway smooth muscle" @default.
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• W1886434626 doi "https://doi.org/10.1152/jappl.2001.91.5.2266" @default.
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