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- W1886475448 abstract "Multiplex PCR assays for respiratory viruses are widely used in routine diagnostics, as they are highly sensitive, rapid, and cost effective. However, depending on the assay system, cross-reactivity between viruses that share a high sequence homology as well as detection of rare virus isolates with sequence variations can be problematic. Virus sequence-independent metagenomic high-throughput sequencing allows for accurate detection of all virus species in a given sample, as we demonstrate here for human Enterovirus and Rhinovirus in a lung transplant patient. While early in infection a commercial PCR assay recorded Rhinovirus, high-throughput sequencing correctly identified human Enterovirus C104 as the source of infection, highlighting the potential of the technology and the benefit of applying open assay formats in complex diagnostic situations." @default.
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- W1886475448 date "2015-10-01" @default.
- W1886475448 modified "2023-10-18" @default.
- W1886475448 title "Unbiased metagenomic sequencing complements specific routine diagnostic methods and increases chances to detect rare viral strains" @default.
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- W1886475448 doi "https://doi.org/10.1016/j.diagmicrobio.2015.06.017" @default.
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