Matches in SemOpenAlex for { <https://semopenalex.org/work/W1886766082> ?p ?o ?g. }
- W1886766082 endingPage "314" @default.
- W1886766082 startingPage "300" @default.
- W1886766082 abstract "Summary We recently identified a gene ( FTN_0818 ) required for F rancisella virulence that seemed likely involved in biotin metabolism. However, the molecular function of this virulence determinant was unclear. Here we show that this protein named BioJ is the enzyme of the biotin biosynthesis pathway that determines the chain length of the biotin valeryl side‐chain. Expression of bioJ allows growth of an E scherichia coli bioH strain on biotin‐free medium, indicating functional equivalence of BioJ to the paradigm pimeloyl‐ ACP methyl ester carboxyl‐esterase, BioH . BioJ was purified to homogeneity, shown to be monomeric and capable of hydrolysis of its physiological substrate methyl pimeloyl‐ ACP to pimeloyl‐ ACP , the precursor required to begin formation of the fused heterocyclic rings of biotin. Phylogenetic analyses confirmed that distinct from BioH , BioJ represents a novel subclade of the α/β‐hydrolase family. Structure‐guided mapping combined with site‐directed mutagenesis revealed that the BioJ catalytic triad consists of Ser151 , Asp248 and His278 , all of which are essential for activity and virulence. The biotin synthesis pathway was reconstituted reaction in vitro and the physiological role of BioJ directly assayed. To the best of our knowledge, these data represent further evidence linking biotin synthesis to bacterial virulence." @default.
- W1886766082 created "2016-06-24" @default.
- W1886766082 creator A5009161164 @default.
- W1886766082 creator A5015400186 @default.
- W1886766082 creator A5043559026 @default.
- W1886766082 creator A5052774470 @default.
- W1886766082 creator A5089162593 @default.
- W1886766082 creator A5091522261 @default.
- W1886766082 date "2013-12-09" @default.
- W1886766082 modified "2023-10-03" @default.
- W1886766082 title "A<i>Francisella</i>virulence factor catalyses an essential reaction of biotin synthesis" @default.
- W1886766082 cites W1553209430 @default.
- W1886766082 cites W1589518473 @default.
- W1886766082 cites W1769277020 @default.
- W1886766082 cites W1844316610 @default.
- W1886766082 cites W1897957811 @default.
- W1886766082 cites W1965958122 @default.
- W1886766082 cites W1970707285 @default.
- W1886766082 cites W1996471792 @default.
- W1886766082 cites W1999701076 @default.
- W1886766082 cites W2006771903 @default.
- W1886766082 cites W2007278412 @default.
- W1886766082 cites W2009576434 @default.
- W1886766082 cites W2013975672 @default.
- W1886766082 cites W2016962924 @default.
- W1886766082 cites W2022537334 @default.
- W1886766082 cites W2023851466 @default.
- W1886766082 cites W2039794827 @default.
- W1886766082 cites W2051889061 @default.
- W1886766082 cites W2053527401 @default.
- W1886766082 cites W2062336984 @default.
- W1886766082 cites W2064882455 @default.
- W1886766082 cites W2066178028 @default.
- W1886766082 cites W2078355424 @default.
- W1886766082 cites W2079722419 @default.
- W1886766082 cites W2082056887 @default.
- W1886766082 cites W2083651925 @default.
- W1886766082 cites W2086328166 @default.
- W1886766082 cites W2088347712 @default.
- W1886766082 cites W2090761475 @default.
- W1886766082 cites W2094823810 @default.
- W1886766082 cites W2098798252 @default.
- W1886766082 cites W2099340095 @default.
- W1886766082 cites W2102004106 @default.
- W1886766082 cites W2102789902 @default.
- W1886766082 cites W2108242412 @default.
- W1886766082 cites W2109491705 @default.
- W1886766082 cites W2110903052 @default.
- W1886766082 cites W2114216552 @default.
- W1886766082 cites W2114560004 @default.
- W1886766082 cites W2117041996 @default.
- W1886766082 cites W2127185441 @default.
- W1886766082 cites W2136335248 @default.
- W1886766082 cites W2140794624 @default.
- W1886766082 cites W2141515959 @default.
- W1886766082 cites W2144175569 @default.
- W1886766082 cites W2144594929 @default.
- W1886766082 cites W2146469860 @default.
- W1886766082 cites W2150958856 @default.
- W1886766082 cites W2155350392 @default.
- W1886766082 cites W2159682090 @default.
- W1886766082 cites W2171780940 @default.
- W1886766082 cites W4244086993 @default.
- W1886766082 doi "https://doi.org/10.1111/mmi.12460" @default.
- W1886766082 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3933004" @default.
- W1886766082 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24313380" @default.
- W1886766082 hasPublicationYear "2013" @default.
- W1886766082 type Work @default.
- W1886766082 sameAs 1886766082 @default.
- W1886766082 citedByCount "57" @default.
- W1886766082 countsByYear W18867660822014 @default.
- W1886766082 countsByYear W18867660822015 @default.
- W1886766082 countsByYear W18867660822016 @default.
- W1886766082 countsByYear W18867660822017 @default.
- W1886766082 countsByYear W18867660822018 @default.
- W1886766082 countsByYear W18867660822019 @default.
- W1886766082 countsByYear W18867660822020 @default.
- W1886766082 countsByYear W18867660822021 @default.
- W1886766082 countsByYear W18867660822022 @default.
- W1886766082 countsByYear W18867660822023 @default.
- W1886766082 crossrefType "journal-article" @default.
- W1886766082 hasAuthorship W1886766082A5009161164 @default.
- W1886766082 hasAuthorship W1886766082A5015400186 @default.
- W1886766082 hasAuthorship W1886766082A5043559026 @default.
- W1886766082 hasAuthorship W1886766082A5052774470 @default.
- W1886766082 hasAuthorship W1886766082A5089162593 @default.
- W1886766082 hasAuthorship W1886766082A5091522261 @default.
- W1886766082 hasBestOaLocation W18867660821 @default.
- W1886766082 hasConcept C104317684 @default.
- W1886766082 hasConcept C145770059 @default.
- W1886766082 hasConcept C16318435 @default.
- W1886766082 hasConcept C181199279 @default.
- W1886766082 hasConcept C2777261171 @default.
- W1886766082 hasConcept C2778204606 @default.
- W1886766082 hasConcept C2780120296 @default.
- W1886766082 hasConcept C2781090273 @default.
- W1886766082 hasConcept C41183919 @default.
- W1886766082 hasConcept C501734568 @default.