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• W1887860551 abstract "Literature Report1 August 2001free access A novel lectin in the secretory pathway An elegant mechanism for glycoprotein elimination Ineke Braakman Ineke Braakman Search for more papers by this author Ineke Braakman Ineke Braakman Search for more papers by this author Author Information Ineke Braakman EMBO Reports (2001)2:666-668https://doi.org/10.1093/embo-reports/kve162 PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info How important can sugars be? Sure, glycoproteins need their glycans during synthesis for proper folding: the sugar chain increases solubility and prevents aggregation, and it mediates interactions with various proteins. On the other hand, so many different glycans exist on glycoproteins that it is difficult to imagine that each would have a specific function. Still, now and again, a new protein is identified that interacts with a particular carbohydrate chain, and that turns out to be crucial for a particular stage in the biosynthesis and maturation of glycoproteins. Will there be many, or do we have only a few? Whichever the answer, glycobiology is celebrating a certain revival. The first step of N-linked glycosylation is highly conserved amongst eukaryotic organisms: the Glc3Man9GlcNAc2 glycan chain is co-translationally attached to the Asn residue in the consensus motif Asn-X-Ser/Thr (Kornfeld and Kornfeld, 1985). Immediately after attachment, monosaccharides are removed one by one (Figure 1), only to prepare the glycan for the later addition of other monosaccharides. This apparently futile sequence of events is a consequence of the necessary signaling to glycan-specific lectins with various gate-keeper functions in the secretory pathway (Helenius and Aebi, 2001). Figure 1.Modifications of the N-linked glycan on proteins. Glycans are attached as the high mannose Glc3Man9GlcNAc2 chain. Immediately after attachment to the protein, the first glucose residue (triangle) is removed by glucosidase I, the second and third by glucosidase II. The third glucose can be reattached by a glucosyltranferase that recognizes only misfolded and unfolded proteins (Parodi, 2000; Helenius and Aebi, 2001). Calnexin and its soluble family member calreticulin (not depicted) associate with the monoglucose chain to retain immature glycoproteins in the ER, to allow folding to proceed, and to prevent release into the secretory pathway (Helenius et al., 1997; Parodi, 2000; Helenius and Aebi, 2001). The ER α1,2-mannosidase removes a single mannose, resulting in Glc0−3Man8GlcNAc2, before transport of the properly folded protein to the Golgi complex where further processing occurs. The Man8 glycan, containing a yet unknown number of glucose residues, is recognized by Htm1p/EDEM and targeted for degradation. Download figure Download PowerPoint Lectins are defined as non-enzymatic, sugar-binding proteins that lack enzymatic activity towards carbohydrates. Up to now, only four lectins had been identified early in the secretory pathway, each with specific glycan recognition, and each with a specific function. Calnexin and calreticulin act as molecular chaperones in the endoplasmic reticulum (ER; Ellgaard et al., 2000; Parodi, 2000), ERGIC-53 functions as a cargo receptor for transport from the ER to the intermediate compartment between the ER and the Golgi complex (ERGIC; Hauri et al., 2000), and VIP36 may function as a cargo receptor within the cis-Golgi (Fiedler et al., 1994). The presence of a fifth lectin, which targets proteins with an attached Glc0−3Man8GlcNAc2 for degradation, was suggested by several reports (Su et al., 1993; Liu et al., 1997; Jakob et al., 1998; Fagioli and Sitia, 2001). In 1998, Jakob et al. published a detailed study in which they showed that, in Saccharomyces cerevisiae, specifically the Man8GlcNAc2 intermediate allows rapid degradation of the protein to which it is attached (mutant carboxypeptidase Y in this case). This and the other reports prompted the speculation that an ER-localized lectin recognizing Man8GlcNAc2 glycans should be responsible for targeting misfolded and unfolded proteins to the proteasome for degradation. And indeed, three papers now report the identification of α1,2-mannosidase-like proteins in S. cerevisiae and in mouse cells. These two proteins are homologous to one another and lack enzymatic mannosidase activity, suggesting that they are mannose-binding lectins (Hosokawa et al., 2001; Jakob et al., 2001; Nakatsukasa et al., 2001). A thorough biochemical study from the group of Nagata (Hosokawa et al., 2001) shows that the mouse protein EDEM has no mannosidase activity, it localizes to the ER, and it is upregulated upon ER stress. Overexpression of this protein resulted in increased degradation of misfolded α1-antitrypsin, but not of a non-glycosylated protein. As EDEM co-immunoprecipitates with misfolded protein, its role in degradation is more than suggestive. The presence of residues crucial for mannose binding completed the picture of EDEM as a mannose-binding lectin important for ER-associated degradation. The group of Aebi (Jakob et al., 2001) reached similar conclusions about the Htm1 protein by different means. Jakob et al. identified the yeast Htm1 protein via a search for mannosidase homologs. They then used mostly genetic strategies to demonstrate that deletion of the protein from a yeast cell stabilizes glycoproteins but not a non-glycosylated protein. The results of the htm1 deletion were similar to the consequences of gene deletions that prevented the formation of a Man8 intermediate via a defect in either biosynthesis or trimming. The authors therefore suggest that Htm1p acts through Man8. The third report, by the group of Endo (Nakatsukasa et al., 2001), describes the identification of the yeast Mnl1 protein, which is identical to Htm1p. The experiments were similar to those of Hosokawa et al., but were performed in S. cerevisiae. Newly synthesized proteins may reside in the ER for a long time, and folding intermediates must be distinguished from misfolded proteins and removed. Both may be associated with calnexin and calreticulin, which retain them in the ER, but at some point the decision for degradation must be made for proteins that will never reach a properly folded conformation. A specific signal for degradation is needed at this stage. Glycan trimming and the subsequent recognition of particular glycans have been suggested to act as a timer for degradation (Su et al., 1993; Helenius et al., 1997). α1,2-mannosidase, which removes one mannose from Man9, apparently acts slowly, leaving sufficient time for folding. If a protein folds extremely slowly or into the wrong conformation, it ultimately reaches the Man8 state that targets it for degradation. The Man8-related literature indicates degradation periods of hours, compatible with the timer-hypothesis. We cannot, however, assume this mechanism to be simply time-dependent. The normal folding rates of proteins are highly variable, and the α1,2-mannosidase may remove mannose groups on different glycans at rates that differ depending on factors such as accessibility. Ignored in many papers dealing with ER-associated degradation is the substantial amount of breakdown of nascent chains, already during protein synthesis. Hosokawa and colleagues show that 35S metabolic glycoprotein labeling of newly synthesized proteins (including EDEM) is strongly increased when protein degradation is inhibited (Hosokawa et al., 2001), suggesting a co-translational effect of the inhibitors. Mannosidase inhibition, which prevents removal of the ninth mannose residue, and hence the formation of the Man8 glycan, also increased labeling, indicating an at least partially co-translational degradation mediated by mannose trimming. Proteasome inhibitors only mildly inhibited degradation induced by overexpression of EDEM. Thus, an additional, alternative degradation pathway is likely to exist. Contrasting with the timer hypothesis, which requires slow mannosidase activity, co-translational degradation mediated by the Man8 lectin must be rapid, taking place within the minutes it takes to synthesize a protein of average size. Since EDEM contains five N-linked glycans, it may be subject to the same mannose-mediated control as the proteins it regulates. The argument for Man8-lectin triggering protein degradation is very convincing, and Htm1p/EDEM seems to play a role in this process. On the other hand, Man8 cannot be necessary or sufficient. Alternative pathways of glycan trimming may exist in different cell types and species (Herscovics, 1999), and many glycoproteins that move successfully to the Golgi from the ER will have had a Man8 intermediate. Perhaps the enzyme that checks the conformational quality of proteins earlier in the ER, the UDP-glucose:glycoprotein glucosyltransferase (Parodi, 2000), attaches a glucose to the Man8 glycan as well, and provides the specific signal for degradation. This would require Htm1p/EDEM to recognize only Glc1Man8 glycans and not any other Man8 glycans. An alternative scenario would predict that Htm1p/EDEM is not purely a lectin, but recognizes misfolded protein stretches as well. Now that one lectin with a role in targeting proteins for degradation has been identified, it is tempting to speculate that more will follow. Candidates are lectins recognizing the Man9 or Man5 intermediate, alternative Man8 forms, and perhaps even every other common intermediate in the trimming pathway. The papers on Htm1p/Mnl1p and EDEM signify only the next step in the long, hard search for the diverse roles of sugars in cell biology. Acknowledgements I thank Peter van der Sluijs for critically reading the manuscript, and members of my group for support and discussions. Biography The author is at the Department of Bio-Organic Chemistry, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands Tel: +31 30 253 2759/2184; Fax: +31 30 253 6900; E-mail: [email protected] References Ellgaard L., Molinari M. and Helenius A. (2000) Setting the standards: Quality control in the secretory pathway. Science, 286, 1882–1888.CrossrefPubMedWeb of Science®Google Scholar Fagioli C. and Sitia R. (2001) Glycoprotein quality control in the endoplasmic reticulum. J. Biol. Chem., 276, 12885–12892.CrossrefCASPubMedWeb of Science®Google Scholar Fiedler K., Parton R.G., Kellner R., Etzold T. and Simons K. (1994) VIP36, a novel component of glycolipid rafts and exocytic carrier vesicles in epithelial cells. EMBO J., 13, 1729–1740.Wiley Online LibraryCASPubMedWeb of Science®Google Scholar Hauri H., Appenzeller C., Kuhn F. and Nufer O. (2000) Lectins and traffic in the secretory pathway. FEBS Lett., 476, 32–37.Wiley Online LibraryCASPubMedWeb of Science®Google Scholar Helenius A. and Aebi M. (2001) Intracellular functions of N-linked glycans. Science, 291, 2364–2369.CrossrefCASPubMedWeb of Science®Google Scholar Helenius A., Trombetta E.S., Hebert D.N. and Simons J.F. (1997) Calnexin, calreticulin and the folding of glycoproteins. Trends Cell Biol., 7, 193–200.CrossrefCASPubMedWeb of Science®Google Scholar Herscovics A. (1999) Importance of glycosidases in mammalian glycoprotein biosynthesis. Biochim. Biophys. Acta, 1473, 96–107.CrossrefCASPubMedWeb of Science®Google Scholar Hosokawa N., Wada I., Hasegawa K., Yorihuzi T., Tremblay L.O., Herscovics A. and Nagata K. (2001) A novel ER α-mannosidase-like protein accelerates ER-associated degradation. EMBO Rep., 2, 415–422.Wiley Online LibraryCASPubMedWeb of Science®Google Scholar Jakob C.A., Bodmer D., Spirig U., Bättig P., Marcil A., Dignard D., Bergeron J.J.M., Thomas D.Y. and Aebi M. (2001) Htm1p, a mannosidase-like protein, is involved in glycoprotein degradation in yeast. EMBO Rep., 2, 423–430.Wiley Online LibraryCASPubMedWeb of Science®Google Scholar Jakob C.A., Burda P., Roth J. and Aebi M. (1998) Degradation of misfolded endoplasmic reticulum glycoproteins in Saccharomyces cerevisiae is determined by a specific oligosaccharide structure. J. Cell Biol., 142, 1223–1233.CrossrefCASPubMedWeb of Science®Google Scholar Kornfeld R. and Kornfeld S. (1985) Assembly of asparagine-linked oligosaccharides. Annu. Rev. Biochem., 54, 631–664.CrossrefCASPubMedWeb of Science®Google Scholar Liu Y., Choudhury P., Cabral C.M. and Sifers R.N. (1997) Intracellular disposal of incompletely folded human α1-antitrypsin involves release from calnexin and post-translational trimming of asparagine-linked oligosaccharides. J. Biol. Chem., 272, 7946–7951.CrossrefCASPubMedWeb of Science®Google Scholar Nakatsukasa K., Nishikawa S., Hosokawa N., Nagata K. and Endo T. (2001) Mnl1p, an α-mannosidase-like protein in yeast Saccharomyces cerevisiae, is required for endoplasmic reticulum-associated degradation of glycoproteins. J. Biol. Chem., 276, 8635–8638.CrossrefCASPubMedWeb of Science®Google Scholar Parodi A.J. (2000) Protein glucosylation and its role in protein folding. Annu. Rev. Biochem., 69, 69–93.CrossrefCASPubMedWeb of Science®Google Scholar Su K., Stoller T., Rocco J., Zemsky J. and Green R. (1993) Pre-Golgi degradation of yeast prepro-α-factor in a mammalian cell. J. Biol. Chem., 268, 14301–14309.CASPubMedWeb of Science®Google Scholar Previous ArticleNext Article Volume 2Issue 81 August 2001In this issue FiguresReferencesRelatedDetailsLoading ..." @default.
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