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• W1888361025 startingPage "2726" @default.
• W1888361025 abstract "To be efficiently expressed in vivo, the vaccinia virus late gene, L65, requires 5'-proximal cis-acting elements which bind a factor from infected cells. Deletion mutagenesis and vaccinia virus helper-dependent transient expression procedures were used to demonstrate that two distinct late promoter elements direct transcription from two different start sites (proximal [+1] and distal [-92]). The -128 to -112 region was essential for L65 distal promoter function, while sequences between -59 and +50 were sufficient for L65 proximal promoter function. The proximal DNA sequences interact with a protein, binding factor I (BF-I), which was isolated and partially purified from vaccinia virus-infected cells at late times postinfection. This activity is not detectable in uninfected cells or in purified virions. This factor binds specifically to two different sites within the proximal promoter, one 5' and one 3' to the transcription start site, but does not bind to the distal promoter element." @default.
• W1888361025 created "2016-06-24" @default.
• W1888361025 creator A5075893471 @default.
• W1888361025 creator A5082551727 @default.
• W1888361025 date "1989-06-01" @default.
• W1888361025 modified "2023-10-16" @default.
• W1888361025 title "DNA sequences that regulate expression of a vaccinia virus late gene (L65) and interact with a DNA-binding protein from infected cells" @default.
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• W1888361025 doi "https://doi.org/10.1128/jvi.63.6.2726-2736.1989" @default.
• W1888361025 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/250768" @default.
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