FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1888767532> ?p ?o ?g. }

• W1888767532 endingPage "6244" @default.
• W1888767532 startingPage "6239" @default.
• W1888767532 abstract "Cobyrinic acid a,c-diamide synthase, which catalyzes the conversion of cobyrinic acid to cobyrinic acid a,c-diamide via the intermediate formation of cobyrinic acid c-monoamide, was purified 155-fold to homogeneity from extracts of a recombinant strain of Pseudomonas denitrificans by high-performance liquid chromatography. The enzyme has an apparent molecular weight of 86,000 and consists of two identical subunits of Mr 45,000, as estimated by gel electrophoresis under denaturing conditions. Stepwise Edman degradation provided the N-terminal sequence of the first 15 amino acids. Glutamine was shown to be the preferred amino group donor (Km = 20.3 microM), but it could be replaced by ammonia (Km = 12 mM). The reaction was ATP dependent and exhibited a broad optimum pH around 7.3. Km values for (CN,aq)cobyrinic acid, (aq)2cobyrinic acid, and (CN,aq)cobyrinic acid c-monoamide were 160, greater than or equal to 250, and 71 microM, respectively. Hydrogenobyrinic acid and hydrogenobyrinic acid c-monoamide were shown to be much better substrates, with Km values of 0.41 and 0.21 microM, respectively." @default.
• W1888767532 created "2016-06-24" @default.
• W1888767532 creator A5017605992 @default.
• W1888767532 creator A5019871610 @default.
• W1888767532 creator A5028945706 @default.
• W1888767532 creator A5035028280 @default.
• W1888767532 creator A5052089394 @default.
• W1888767532 date "1990-11-01" @default.
• W1888767532 modified "2023-10-13" @default.
• W1888767532 title "Purification and characterization of cobyrinic acid a,c-diamide synthase from Pseudomonas denitrificans" @default.
• W1888767532 cites W129910819 @default.
• W1888767532 cites W1510702873 @default.
• W1888767532 cites W1528030144 @default.
• W1888767532 cites W1534471640 @default.
• W1888767532 cites W1584578721 @default.
• W1888767532 cites W1599809900 @default.
• W1888767532 cites W1761046574 @default.
• W1888767532 cites W1924086082 @default.
• W1888767532 cites W1965986646 @default.
• W1888767532 cites W1973588098 @default.
• W1888767532 cites W2039704404 @default.
• W1888767532 cites W2040512686 @default.
• W1888767532 cites W2082972937 @default.
• W1888767532 cites W2115934423 @default.
• W1888767532 cites W2163793292 @default.
• W1888767532 cites W2172818991 @default.
• W1888767532 cites W2204880251 @default.
• W1888767532 cites W2255220704 @default.
• W1888767532 cites W2394904417 @default.
• W1888767532 cites W2397741733 @default.
• W1888767532 cites W2408774066 @default.
• W1888767532 cites W2408840327 @default.
• W1888767532 cites W2413738699 @default.
• W1888767532 cites W2416943896 @default.
• W1888767532 cites W2992098703 @default.
• W1888767532 cites W2992735005 @default.
• W1888767532 doi "https://doi.org/10.1128/jb.172.11.6239-6244.1990" @default.
• W1888767532 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/526805" @default.
• W1888767532 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2172209" @default.
• W1888767532 hasPublicationYear "1990" @default.
• W1888767532 type Work @default.
• W1888767532 sameAs 1888767532 @default.
• W1888767532 citedByCount "53" @default.
• W1888767532 countsByYear W18887675322014 @default.
• W1888767532 countsByYear W18887675322018 @default.
• W1888767532 countsByYear W18887675322020 @default.
• W1888767532 countsByYear W18887675322021 @default.
• W1888767532 countsByYear W18887675322023 @default.
• W1888767532 crossrefType "journal-article" @default.
• W1888767532 hasAuthorship W1888767532A5017605992 @default.
• W1888767532 hasAuthorship W1888767532A5019871610 @default.
• W1888767532 hasAuthorship W1888767532A5028945706 @default.
• W1888767532 hasAuthorship W1888767532A5035028280 @default.
• W1888767532 hasAuthorship W1888767532A5052089394 @default.
• W1888767532 hasBestOaLocation W18887675321 @default.
• W1888767532 hasConcept C104317684 @default.
• W1888767532 hasConcept C167625842 @default.
• W1888767532 hasConcept C181199279 @default.
• W1888767532 hasConcept C185592680 @default.
• W1888767532 hasConcept C2779349466 @default.
• W1888767532 hasConcept C2781463711 @default.
• W1888767532 hasConcept C43617362 @default.
• W1888767532 hasConcept C515207424 @default.
• W1888767532 hasConcept C523546767 @default.
• W1888767532 hasConcept C54355233 @default.
• W1888767532 hasConcept C55493867 @default.
• W1888767532 hasConcept C71240020 @default.
• W1888767532 hasConcept C86803240 @default.
• W1888767532 hasConcept C87933860 @default.
• W1888767532 hasConceptScore W1888767532C104317684 @default.
• W1888767532 hasConceptScore W1888767532C167625842 @default.
• W1888767532 hasConceptScore W1888767532C181199279 @default.
• W1888767532 hasConceptScore W1888767532C185592680 @default.
• W1888767532 hasConceptScore W1888767532C2779349466 @default.
• W1888767532 hasConceptScore W1888767532C2781463711 @default.
• W1888767532 hasConceptScore W1888767532C43617362 @default.
• W1888767532 hasConceptScore W1888767532C515207424 @default.
• W1888767532 hasConceptScore W1888767532C523546767 @default.
• W1888767532 hasConceptScore W1888767532C54355233 @default.
• W1888767532 hasConceptScore W1888767532C55493867 @default.
• W1888767532 hasConceptScore W1888767532C71240020 @default.
• W1888767532 hasConceptScore W1888767532C86803240 @default.
• W1888767532 hasConceptScore W1888767532C87933860 @default.
• W1888767532 hasIssue "11" @default.
• W1888767532 hasLocation W18887675321 @default.
• W1888767532 hasLocation W18887675322 @default.
• W1888767532 hasLocation W18887675323 @default.
• W1888767532 hasLocation W18887675324 @default.
• W1888767532 hasOpenAccess W1888767532 @default.
• W1888767532 hasPrimaryLocation W18887675321 @default.
• W1888767532 hasRelatedWork W1697966926 @default.
• W1888767532 hasRelatedWork W1988600174 @default.
• W1888767532 hasRelatedWork W2015069262 @default.
• W1888767532 hasRelatedWork W2016062340 @default.
• W1888767532 hasRelatedWork W2023660858 @default.
• W1888767532 hasRelatedWork W2058609912 @default.
• W1888767532 hasRelatedWork W2073824547 @default.
• W1888767532 hasRelatedWork W2075693051 @default.

• next