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- W1888794300 abstract "In this study, we investigated hepatitis E virus (HEV) infection in piglets sampled in two farms in southern Italy. The virus was detected in 11 out of 15 animals tested. Based on sequence analysis, the 6 Italian strains examined belonged to two clusters containing both swine and human strains of either genotype 3 subtype e or f from Europe and Japan. The two Italian strain clusters shared nucleotide identity of 81.8% and 87.5% in the ORF2 (capsid protein) and ORF1 (RdRp) diagnostic fragments, respectively, confirming the heterogeneity of genotype 3 viruses circulating in pigs in Italy. The complete genome of one genotype 3 subtype e strain and the full ORF2 and ORF3 coding regions of one of the genotype 3f strains, obtained in this study, were compared to other HEV sequences available on line (NCBI database). The results of analysis showed that porcine strains clustered together with human and swine strains detected in Europe. Most changes in the coding region corresponded to synonymous mutations, and only the ORF3 showed a positive selection. Further, analyses are needed to understand the clinical significance of HEV genotypes and subtypes." @default.
- W1888794300 created "2016-06-24" @default.
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- W1888794300 date "2016-01-01" @default.
- W1888794300 modified "2023-09-23" @default.
- W1888794300 title "Complete genome sequencing of a genotype 3 hepatitis E virus strain identified in a swine farm in Italy" @default.
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- W1888794300 doi "https://doi.org/10.1016/j.virusres.2015.09.014" @default.
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