Matches in SemOpenAlex for { <https://semopenalex.org/work/W1889247925> ?p ?o ?g. }
Showing items 1 to 71 of
71
with 100 items per page.
- W1889247925 endingPage "412" @default.
- W1889247925 startingPage "403" @default.
- W1889247925 abstract "Bovine erythrocytes were treated with each of three bacterial phospholipases C; phosphatidylcholine-hydrolyzing phospholipase C (PCase) of Clostridium perfringens, sphingomyelinase C (SMase) of Bacillus cereus and phosphatidylinositol-specific phospholipase C (PIase) of Bacillus thuringiensis. An increase in osmotic fragility was detected by means of a coil planet centrifugation (CPC) apparatus (Biomedical Systems Co., Tokyo) after the treatment with these enzymes. The peak of hemolysis normally observed in the untreated erythrocytes at the range between 50 and 100 mOsM shifted to 160 to 200 mOsM with the progress of sphingomyelin hydrolysis by phospholipase C of C. perfringens. Sphingomyelinase C of B. cereus showed two different effects on bovine erythrocytes: In the absence of divalent cations or in the presence of Ca2+ alone, the peak of hemolysis shifted to the region from 130 to 160 mOsM, without appreciable hydrolysis of sphingomyelin, while in the presence of Mg2+ or Mg2+ plus Ca2+, the peak of hemolysis further shifted to the region from 160 to 200 mOsM with the hydrolysis of sphingomyelin. Abrupt shift in osmotic fragility to a much higher region around 250 mOsM was produced by treatment with increasing amounts of phosphatidylinositol-specific phospholipase C. In this case, a significant amount of acetylcholinesterase was released from the erythrocyte membrane without hot or hot-cold hemolysis. The mechanism of alteration of osmotic fragility of bovine erythrocytes by treatment with phospholipases C seems to differ from case to case, depending upon the specific action of each enzyme toward the membrane phospholipids." @default.
- W1889247925 created "2016-06-24" @default.
- W1889247925 creator A5011126050 @default.
- W1889247925 creator A5018941611 @default.
- W1889247925 creator A5029449740 @default.
- W1889247925 creator A5030519900 @default.
- W1889247925 date "1983-02-01" @default.
- W1889247925 modified "2023-09-25" @default.
- W1889247925 title "Increase in Osmotic Fragility of Bovine Erythrocytes Induced by Bacterial Phospholipases C" @default.
- W1889247925 doi "https://doi.org/10.1093/oxfordjournals.jbchem.a134194" @default.
- W1889247925 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6302097" @default.
- W1889247925 hasPublicationYear "1983" @default.
- W1889247925 type Work @default.
- W1889247925 sameAs 1889247925 @default.
- W1889247925 citedByCount "13" @default.
- W1889247925 countsByYear W18892479252012 @default.
- W1889247925 crossrefType "journal-article" @default.
- W1889247925 hasAuthorship W1889247925A5011126050 @default.
- W1889247925 hasAuthorship W1889247925A5018941611 @default.
- W1889247925 hasAuthorship W1889247925A5029449740 @default.
- W1889247925 hasAuthorship W1889247925A5030519900 @default.
- W1889247925 hasConcept C12927208 @default.
- W1889247925 hasConcept C147509754 @default.
- W1889247925 hasConcept C181199279 @default.
- W1889247925 hasConcept C185592680 @default.
- W1889247925 hasConcept C203014093 @default.
- W1889247925 hasConcept C2777118263 @default.
- W1889247925 hasConcept C2778597717 @default.
- W1889247925 hasConcept C2779902561 @default.
- W1889247925 hasConcept C41625074 @default.
- W1889247925 hasConcept C523546767 @default.
- W1889247925 hasConcept C54355233 @default.
- W1889247925 hasConcept C55493867 @default.
- W1889247925 hasConcept C61322309 @default.
- W1889247925 hasConcept C86803240 @default.
- W1889247925 hasConceptScore W1889247925C12927208 @default.
- W1889247925 hasConceptScore W1889247925C147509754 @default.
- W1889247925 hasConceptScore W1889247925C181199279 @default.
- W1889247925 hasConceptScore W1889247925C185592680 @default.
- W1889247925 hasConceptScore W1889247925C203014093 @default.
- W1889247925 hasConceptScore W1889247925C2777118263 @default.
- W1889247925 hasConceptScore W1889247925C2778597717 @default.
- W1889247925 hasConceptScore W1889247925C2779902561 @default.
- W1889247925 hasConceptScore W1889247925C41625074 @default.
- W1889247925 hasConceptScore W1889247925C523546767 @default.
- W1889247925 hasConceptScore W1889247925C54355233 @default.
- W1889247925 hasConceptScore W1889247925C55493867 @default.
- W1889247925 hasConceptScore W1889247925C61322309 @default.
- W1889247925 hasConceptScore W1889247925C86803240 @default.
- W1889247925 hasIssue "2" @default.
- W1889247925 hasLocation W18892479251 @default.
- W1889247925 hasLocation W18892479252 @default.
- W1889247925 hasOpenAccess W1889247925 @default.
- W1889247925 hasPrimaryLocation W18892479251 @default.
- W1889247925 hasRelatedWork W1889247925 @default.
- W1889247925 hasRelatedWork W1975941863 @default.
- W1889247925 hasRelatedWork W2005859080 @default.
- W1889247925 hasRelatedWork W2046182106 @default.
- W1889247925 hasRelatedWork W2112684036 @default.
- W1889247925 hasRelatedWork W2313283666 @default.
- W1889247925 hasRelatedWork W2420053277 @default.
- W1889247925 hasRelatedWork W2952275338 @default.
- W1889247925 hasRelatedWork W6774428 @default.
- W1889247925 hasRelatedWork W250066823 @default.
- W1889247925 hasVolume "93" @default.
- W1889247925 isParatext "false" @default.
- W1889247925 isRetracted "false" @default.
- W1889247925 magId "1889247925" @default.
- W1889247925 workType "article" @default.