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- W189007304 abstract "Rhodopseudomonas palustris uroporphyrinogen I synthetase (URO-S) has been chemically attached to Sepharose 4B and some of its properties have been studied. When 7-8 mg protein/ml activated Sepharose was used, immobilized URO-S retained 45% of the activity of the original soluble preparation, with a coupling yield of 66% after a period of 15 h. Optimal incubation conditions for the activity of gel-enzyme were determined. Unlike the soluble enzyme, the Sepharose-bound URO-S showed a biphasic substrate saturation curve, indicating that a protein conformational change had occurred during the process of immobilization. Immobilized URO-S stored at 4 degrees C for 35 days retained 90% of activity and when repeatedly used, up to 5 times, retained 48% of the original activity. Attachment of URO-S to Sepharose led to an enhanced thermal stability." @default.
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- W189007304 date "1990-06-01" @default.
- W189007304 modified "2023-09-26" @default.
- W189007304 title "Immobilized uroporphyrinogen I synthetase from Rhodopseudomonas palustris." @default.
- W189007304 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2360991" @default.
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