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- W189084792 abstract "This chapter focuses on cell and tissue disintegration in enzymatic analysis. During enzymatic analysis, it is desirable to prepare the living material in a form suitable for the measurements but without altering the structure or the relative amounts of the substances to be analyzed. Generally, distinction is made between disintegration methods used for the assay of high molecular weight compounds and those used for low molecular weight metabolites. If enzyme activity is to be determined, the speed with which the tissue is fixed, disintegrated, and extracted is less important. Any proteolytic decomposition during the disintegration (up to several hours) can usually be ignored. To preserve the structure of enzyme proteins, the extraction is carried out at a low temperature with distilled water, salt solutions, sucrose, periston, dextran, or albumin solutions. To avoid denaturation of protein by the acid produced in tissues with a high rate of glycolysis, the solutions used for extraction should be buffered at a suitable pH. The addition of chelating agents to prevent oxidation, or the addition of cysteine to protect SH groups is also recommended. The tissue is disintegrated by careful homogenization, sometimes with the addition of compounds that act on the membranes." @default.
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- W189084792 date "1965-01-01" @default.
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- W189084792 title "Cell and Tissue Disintegration" @default.
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- W189084792 doi "https://doi.org/10.1016/b978-0-12-395630-9.50010-4" @default.
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