FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1891937459> ?p ?o ?g. }

• W1891937459 endingPage "3283" @default.
• W1891937459 startingPage "3273" @default.
• W1891937459 abstract "B cells have previously been identified as the major hematopoietic cell type harboring latent gammaherpesvirus 68 (gammaHV68) (N. P. Sunil-Chandra, S. Efstathiou, and A. A. Nash, J. Gen. Virol. 73:3275-3279, 1992). However, we have shown that gammaHV68 efficiently establishes latency in B-cell-deficient mice (K. E. Weck, M. L. Barkon, L. I. Yoo, S. H. Speck, and H. W. Virgin, J. Virol. 70:6775-6780, 1996), demonstrating that B cells are not required for gammaHV68 latency. To understand this dichotomy, we determined whether hematopoietic cell types, in addition to B cells, carry latent gammaHV68. We observed a high frequency of cells that reactivate latent gammaHV68 in peritoneal exudate cells (PECs) derived from both B-cell-deficient and normal C57BL/6 mice. PECs were composed primarily of macrophages in B-cell-deficient mice and of macrophages plus B cells in normal C57BL/6 mice. To determine which cells in PECs from C57BL/6 mice carry latent gammaHV68, we developed a limiting-dilution PCR assay to quantitate the frequency of cells carrying the gammaHV68 genome in fluorescence-activated cell sorter-purified cell populations. We also quantitated the contribution of individual cell populations to the total frequency of cells carrying latent gammaHV68. At early times after infection, the frequency of PECs that reactivated gammaHV68 correlated very closely with the frequency of PECs carrying the gammaHV68 genome, validating measurement of the frequency of viral-genome-positive cells as a measure of latency in this cell population. F4/80-positive macrophage-enriched, lymphocyte-depleted PECs harbored most of the gammaHV68 genome and efficiently reactivated gammaHV68, while CD19-positive, B-cell-enriched PECs harbored about a 10-fold lower frequency of gammaHV68 genome-positive cells. CD4-positive, T-cell-enriched PECs contained only a very low frequency of gammaHV68 genome-positive cells, consistent with previous analyses indicating that T cells are not a reservoir for gammaHV68 latency (N. P. Sunil-Chandra, S. Efstathiou, and A. A. Nash, J. Gen. Virol. 73:3275-3279, 1992). Since macrophages are bone marrow derived, we determined whether elicitation of a large inflammatory response in the peritoneum would recruit additional latent cells into the peritoneum. Thioglycolate inoculation increased the total number of PECs by about 20-fold but did not affect the frequency of cells that reactivate gammaHV68, consistent with a bone marrow reservoir for latent gammaHV68. These experiments demonstrate gammaHV68 latency in two different hematopoietic cell types, F4/80-positive macrophages and CD19-positive B cells, and argue for a bone marrow reservoir for latent gammaHV68." @default.
• W1891937459 created "2016-06-24" @default.
• W1891937459 creator A5016561426 @default.
• W1891937459 creator A5018204002 @default.
• W1891937459 creator A5042031712 @default.
• W1891937459 creator A5070627463 @default.
• W1891937459 date "1999-04-01" @default.
• W1891937459 modified "2023-10-14" @default.
• W1891937459 title "Macrophages Are the Major Reservoir of Latent Murine Gammaherpesvirus 68 in Peritoneal Cells" @default.
• W1891937459 cites W1486831857 @default.
• W1891937459 cites W1491026704 @default.
• W1891937459 cites W1502717333 @default.
• W1891937459 cites W1539529772 @default.
• W1891937459 cites W1600662889 @default.
• W1891937459 cites W1672767687 @default.
• W1891937459 cites W1749209363 @default.
• W1891937459 cites W1935097178 @default.
• W1891937459 cites W1974538337 @default.
• W1891937459 cites W1987658986 @default.
• W1891937459 cites W2022659649 @default.
• W1891937459 cites W2028613979 @default.
• W1891937459 cites W2032348735 @default.
• W1891937459 cites W2054989155 @default.
• W1891937459 cites W2057138957 @default.
• W1891937459 cites W2065866793 @default.
• W1891937459 cites W2065871536 @default.
• W1891937459 cites W2070437307 @default.
• W1891937459 cites W2074410605 @default.
• W1891937459 cites W2096179876 @default.
• W1891937459 cites W2103717331 @default.
• W1891937459 cites W2111656409 @default.
• W1891937459 cites W2126982510 @default.
• W1891937459 cites W2128181554 @default.
• W1891937459 cites W2143090686 @default.
• W1891937459 cites W2144326983 @default.
• W1891937459 cites W2146299680 @default.
• W1891937459 cites W2146707443 @default.
• W1891937459 cites W2168415907 @default.
• W1891937459 cites W2170401763 @default.
• W1891937459 cites W2188464094 @default.
• W1891937459 cites W2292695347 @default.
• W1891937459 cites W2336241188 @default.
• W1891937459 cites W2413722345 @default.
• W1891937459 cites W2417441042 @default.
• W1891937459 doi "https://doi.org/10.1128/jvi.73.4.3273-3283.1999" @default.
• W1891937459 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/104091" @default.
• W1891937459 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10074181" @default.
• W1891937459 hasPublicationYear "1999" @default.
• W1891937459 type Work @default.
• W1891937459 sameAs 1891937459 @default.
• W1891937459 citedByCount "276" @default.
• W1891937459 countsByYear W18919374592012 @default.
• W1891937459 countsByYear W18919374592013 @default.
• W1891937459 countsByYear W18919374592014 @default.
• W1891937459 countsByYear W18919374592015 @default.
• W1891937459 countsByYear W18919374592016 @default.
• W1891937459 countsByYear W18919374592017 @default.
• W1891937459 countsByYear W18919374592018 @default.
• W1891937459 countsByYear W18919374592019 @default.
• W1891937459 countsByYear W18919374592020 @default.
• W1891937459 countsByYear W18919374592021 @default.
• W1891937459 countsByYear W18919374592022 @default.
• W1891937459 countsByYear W18919374592023 @default.
• W1891937459 crossrefType "journal-article" @default.
• W1891937459 hasAuthorship W1891937459A5016561426 @default.
• W1891937459 hasAuthorship W1891937459A5018204002 @default.
• W1891937459 hasAuthorship W1891937459A5042031712 @default.
• W1891937459 hasAuthorship W1891937459A5070627463 @default.
• W1891937459 hasBestOaLocation W18919374591 @default.
• W1891937459 hasConcept C109159458 @default.
• W1891937459 hasConcept C140704245 @default.
• W1891937459 hasConcept C1491633281 @default.
• W1891937459 hasConcept C153911025 @default.
• W1891937459 hasConcept C159047783 @default.
• W1891937459 hasConcept C159654299 @default.
• W1891937459 hasConcept C203014093 @default.
• W1891937459 hasConcept C2522874641 @default.
• W1891937459 hasConcept C2778453870 @default.
• W1891937459 hasConcept C2778852064 @default.
• W1891937459 hasConcept C28328180 @default.
• W1891937459 hasConcept C2908647359 @default.
• W1891937459 hasConcept C54355233 @default.
• W1891937459 hasConcept C71924100 @default.
• W1891937459 hasConcept C86803240 @default.
• W1891937459 hasConcept C95444343 @default.
• W1891937459 hasConcept C99454951 @default.
• W1891937459 hasConceptScore W1891937459C109159458 @default.
• W1891937459 hasConceptScore W1891937459C140704245 @default.
• W1891937459 hasConceptScore W1891937459C1491633281 @default.
• W1891937459 hasConceptScore W1891937459C153911025 @default.
• W1891937459 hasConceptScore W1891937459C159047783 @default.
• W1891937459 hasConceptScore W1891937459C159654299 @default.
• W1891937459 hasConceptScore W1891937459C203014093 @default.
• W1891937459 hasConceptScore W1891937459C2522874641 @default.
• W1891937459 hasConceptScore W1891937459C2778453870 @default.
• W1891937459 hasConceptScore W1891937459C2778852064 @default.
• W1891937459 hasConceptScore W1891937459C28328180 @default.
• W1891937459 hasConceptScore W1891937459C2908647359 @default.

• next