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- W189362379 abstract "This chapter describes the adenosine triphosphate-binding cassette (ABC) transporters. Mitochondria are the descendants of a bacterial endosymbiont. Given the large number of ABC transporters that have been found in bacteria, it is understood that mitochondria do not themselves retain their own ABC transporters. Mitochondria are metabolically highly active organelles involved in continuous substrate exchange with the rest of the cell. Polymerase chain reactions (PCRs) are used for searching new ABC genes in the yeast genome to identify those that code for mitochondrial proteins. PCR reactions are performed in a volume of 100μl, containing 10 ng of yeast genomic DNA, 200μM of each dNTP, 10μl of a standard 10× PCR buffer (100 mM Tris-HC1, 15 mM MgC12, 500 mM KC1, 1 mg/ml gelatin, pH 8.3) and pooled upstream and downstream oligonucleotide primers at concentrations such that 5 pmol of each unique oligonucleotide is present. The advantage of this approach is that gene fragments can be obtained quickly and immediately for further experiments." @default.
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- W189362379 date "1998-01-01" @default.
- W189362379 modified "2023-10-16" @default.
- W189362379 title "[57] Mitochondrial ABC transporters" @default.
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- W189362379 doi "https://doi.org/10.1016/s0076-6879(98)92059-6" @default.
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