FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1894690623> ?p ?o ?g. }

• W1894690623 endingPage "4208" @default.
• W1894690623 startingPage "4199" @default.
• W1894690623 abstract "We have used a minigene construct of the herpes simplex virus type 1 (HSV-1) latency-associated transcript (LAT) gene to analyze its transcripts in transient transfection assays. A 2.8-kb fragment of the approximately 8.5-kb LAT gene encompassing the 2.0-kb LAT was cloned into a eukaryotic expression vector downstream of the cytomegalovirus immediate-early gene promoter. Northern hybridization of RNA isolated from transfected COS-1 cells identified three LAT-specific transcripts, 3.4, 2.0, and 1.4 kb in size. Mapping of these transcripts by Northern hybridization indicated that the 1.4- and 2.0-kb RNAs are nonoverlapping, while the 3.4-kb RNA overlaps both smaller RNAs. Reverse transcription-PCR (RT-PCR) and partial sequencing of the 1.4-kb RNA revealed that this RNA is the spliced exons of the 3.4-kb primary transcript. The 2.0-kb LAT appears to be an intron accumulating after splicing of the minor LAT (mLAT) pre-mRNA. The splice donor and acceptor sites for the 2.0-kb LAT identified in transfected and HSV-1-infected cells are identical. Mapping of the branch point of this intron by RT-PCR in transfected and HSV-1-infected cells, as well as in latently infected murine trigemial ganglia, shows that it is a guanosine. This branch site does not bear homology to consensus mammalian branch site sequences. These data provide evidence that the 2.0-kb LAT is an intron of the mLAT pre-mRNA with a unique branch point." @default.
• W1894690623 created "2016-06-24" @default.
• W1894690623 creator A5035866436 @default.
• W1894690623 creator A5057971828 @default.
• W1894690623 creator A5070694339 @default.
• W1894690623 date "1997-06-01" @default.
• W1894690623 modified "2023-09-27" @default.
• W1894690623 title "The herpes simplex virus type 1 2.0-kilobase latency-associated transcript is a stable intron which branches at a guanosine" @default.
• W1894690623 cites W1491477100 @default.
• W1894690623 cites W1502566225 @default.
• W1894690623 cites W1505196920 @default.
• W1894690623 cites W1508424174 @default.
• W1894690623 cites W1535530714 @default.
• W1894690623 cites W1535683449 @default.
• W1894690623 cites W1573356806 @default.
• W1894690623 cites W1667878902 @default.
• W1894690623 cites W170486316 @default.
• W1894690623 cites W1733825645 @default.
• W1894690623 cites W1778631963 @default.
• W1894690623 cites W1890282385 @default.
• W1894690623 cites W1914628750 @default.
• W1894690623 cites W1956882355 @default.
• W1894690623 cites W1961266051 @default.
• W1894690623 cites W1964678759 @default.
• W1894690623 cites W1981531064 @default.
• W1894690623 cites W1981956038 @default.
• W1894690623 cites W1986562596 @default.
• W1894690623 cites W1988185664 @default.
• W1894690623 cites W1991055245 @default.
• W1894690623 cites W2000371296 @default.
• W1894690623 cites W2008398524 @default.
• W1894690623 cites W2018809048 @default.
• W1894690623 cites W2027686087 @default.
• W1894690623 cites W2030420103 @default.
• W1894690623 cites W2035136477 @default.
• W1894690623 cites W2036301707 @default.
• W1894690623 cites W2036969473 @default.
• W1894690623 cites W2044339550 @default.
• W1894690623 cites W2056650797 @default.
• W1894690623 cites W2060333964 @default.
• W1894690623 cites W2062829769 @default.
• W1894690623 cites W2070675696 @default.
• W1894690623 cites W2076547039 @default.
• W1894690623 cites W2078678253 @default.
• W1894690623 cites W2081373819 @default.
• W1894690623 cites W2081710371 @default.
• W1894690623 cites W2085924046 @default.
• W1894690623 cites W2097025484 @default.
• W1894690623 cites W2098190039 @default.
• W1894690623 cites W2101202684 @default.
• W1894690623 cites W2104845462 @default.
• W1894690623 cites W2110120221 @default.
• W1894690623 cites W2114568757 @default.
• W1894690623 cites W2115736764 @default.
• W1894690623 cites W2123903713 @default.
• W1894690623 cites W2127157516 @default.
• W1894690623 cites W2127996327 @default.
• W1894690623 cites W2140178603 @default.
• W1894690623 cites W2144382758 @default.
• W1894690623 cites W2145088688 @default.
• W1894690623 cites W2146702776 @default.
• W1894690623 cites W2147990979 @default.
• W1894690623 cites W2149651585 @default.
• W1894690623 cites W2149757236 @default.
• W1894690623 cites W2151786769 @default.
• W1894690623 cites W2153405631 @default.
• W1894690623 cites W2154442938 @default.
• W1894690623 cites W2156388300 @default.
• W1894690623 cites W2161028421 @default.
• W1894690623 cites W2162751422 @default.
• W1894690623 cites W2171349148 @default.
• W1894690623 cites W2467571305 @default.
• W1894690623 cites W4251106005 @default.
• W1894690623 doi "https://doi.org/10.1128/jvi.71.6.4199-4208.1997" @default.
• W1894690623 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/191634" @default.
• W1894690623 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9151806" @default.
• W1894690623 hasPublicationYear "1997" @default.
• W1894690623 type Work @default.
• W1894690623 sameAs 1894690623 @default.
• W1894690623 citedByCount "75" @default.
• W1894690623 countsByYear W18946906232012 @default.
• W1894690623 countsByYear W18946906232013 @default.
• W1894690623 countsByYear W18946906232014 @default.
• W1894690623 countsByYear W18946906232015 @default.
• W1894690623 countsByYear W18946906232016 @default.
• W1894690623 countsByYear W18946906232017 @default.
• W1894690623 countsByYear W18946906232018 @default.
• W1894690623 countsByYear W18946906232019 @default.
• W1894690623 countsByYear W18946906232020 @default.
• W1894690623 countsByYear W18946906232021 @default.
• W1894690623 countsByYear W18946906232023 @default.
• W1894690623 crossrefType "journal-article" @default.
• W1894690623 hasAuthorship W1894690623A5035866436 @default.
• W1894690623 hasAuthorship W1894690623A5057971828 @default.
• W1894690623 hasAuthorship W1894690623A5070694339 @default.
• W1894690623 hasBestOaLocation W18946906231 @default.
• W1894690623 hasConcept C104317684 @default.
• W1894690623 hasConcept C153911025 @default.

• next