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• W1894838379 abstract "Abstract Mammalian cells are widely used in the production of viral vaccines and therapeutic proteins. Most of those processes employ cells growing in suspension; however, some, especially those in vaccine production, use adherent cells. Stirred‐tank bioreactors are most widely used for mammalian cell culture processes. When a stirred‐tank bioreactor is used for growing adherent cells, a supporting surface for cell attachment is provided by the use of microcarriers or macroporous microcarriers. Alternatively, adherent cells are grown as aggregates in suspension. Fed‐batch culture is commonly practised since it reaches high cell and product concentrations. When a stirred‐tank bioreactor is operated in a continuous mode, it is a common practice to employ a cell retention device for perfusion in order to increase cell and product concentrations. As mammalian cell culture processes are increasingly being used in the manufacturing, one also witnesses an increasing adoption of disposable (or single‐use) cell culture devices. Traditional roller bottles are still often seen. Additionally, multiple flat plates or parallel trays, along with bag type cell growth chambers and even plastic stirred tanks, are used in moderate‐scale production and in the seed culture propagation for large‐scale bioreactors. Those disposable devices offer simplicity in operations and ease process validation in the production of biopharmaceuticals. Recently, some innovative bioreactors developed nearly two decades ago are finding applications in tissue engineering. Furthermore, the demand for high throughput operations in cell culture processing will continue to drive the innovations in bioreactor technology." @default.
• W1894838379 created "2016-06-24" @default.
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• W1894838379 date "2010-04-15" @default.
• W1894838379 modified "2023-10-18" @default.
• W1894838379 title "Mammalian Cell Bioreactors" @default.
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• W1894838379 doi "https://doi.org/10.1002/9780470054581.eib394" @default.
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