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• W1894981907 abstract "We investigated the effects of Bordetella bronchiseptica dermonecrotic toxin on DNA synthesis in MC3T3-E1 cells. The rate of [methyl-3H]thymidine incorporation increased in the toxin-treated cells more than 24 h after addition of the toxin under the serum-starved conditions. This effect was dependent on the toxin concentration ranging from 0.3 to 3 ng/ml and was eliminated by aphidicolin and hydroxyurea, inhibitors for DNA replication. In the toxin-treated culture, the number of cells did not increase but polynucleated cells appeared and their number increased to ca. 50% of the total number of cells 6 days after the toxin addition. From these results, we concluded that the toxin stimulates DNA replication in MC3T3-E1 cells without cell proliferation." @default.
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• W1894981907 date "1993-09-01" @default.
• W1894981907 modified "2023-09-30" @default.
• W1894981907 title "Stimulation of DNA synthesis in osteoblast-like MC3T3-E1 cells by Bordetella bronchiseptica dermonecrotic toxin" @default.
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• W1894981907 doi "https://doi.org/10.1128/iai.61.9.3611-3615.1993" @default.
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