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• W1896548460 abstract "Vibrio cholerae, the causative agent of cholera, remains a threat to public health in areas with inadequate sanitation. As a waterborne pathogen, V. cholerae moves between two dissimilar environments, aquatic reservoirs and the intestinal tract of humans. Accordingly, this pathogen undergoes adaptive shifts in gene expression throughout the different stages of its lifecycle. One particular gene, xds, encodes a secreted exonuclease that was previously identified as being induced during infection. Here we sought to identify regulators responsible for the in vivo-specific induction of xds. A transcriptional fusion of xds to two consecutive antibiotic resistance genes was used to select transposon mutants that had inserted within or adjacent to regulatory genes and thereby caused increased expression of the xds fusion under non-inducing conditions. Large pools of selected insertion sites were sequenced in a high throughput manner using Tn-seq to identify potential mechanisms of xds regulation. Our selection identified the two-component system PhoB/R as the dominant activator of xds expression. In vitro validation confirmed that PhoB, a protein which is only active during phosphate limitation, was responsible for xds activation. Using xds expression as a biosensor of the extracellular phosphate level, we observed that the mouse small intestine is a phosphate-limited environment." @default.
• W1896548460 created "2016-06-24" @default.
• W1896548460 creator A5009524336 @default.
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• W1896548460 date "2014-03-14" @default.
• W1896548460 modified "2023-10-12" @default.
• W1896548460 title "Identification of<i>in vivo</i>regulators of the<i>V</i><i>ibrio cholerae</i> <i>xds</i>gene using a high-throughput genetic selection" @default.
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• W1896548460 doi "https://doi.org/10.1111/mmi.12557" @default.
• W1896548460 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4005888" @default.
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• W1896548460 hasPublicationYear "2014" @default.
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