Matches in SemOpenAlex for { <https://semopenalex.org/work/W1897131549> ?p ?o ?g. }
Showing items 1 to 98 of
98
with 100 items per page.
- W1897131549 endingPage "1931" @default.
- W1897131549 startingPage "1924" @default.
- W1897131549 abstract "MS Binding Assays are a label-free alternative to radioligand binding assays. They provide basically the same capabilities as the latter, but an unlabeled reporter ligand is used instead of a radioligand. The study presented herein describes the development of MS Binding Assays that address D1 and D5 dopamine receptors. A highly sensitive, rapid and robust LC-ESI-MS/MS quantification method for the selective D1 dopamine receptor antagonist SCH23390 ((5R)-8-chloro-3-methyl-5-phenyl-1,2,4,5-tetrahydro-3-benzazepin-7-ol) was established and validated, using its 8-bromo analogue SKF83566 as an internal standard. This quantification method proved to be suitable for the characterization of SCH23390 binding to human D1 and D5 receptors. Following the concept of MS Binding Assays, saturation experiments for D1 and D5 receptors were performed, as well as competition experiments for D1 receptors. The results obtained are in good agreement with results from radioligand binding assays and therefore indicate that the established MS Binding Assays addressing D1 and D5 receptors are well-suited substitutes for radioligand binding assays, the technique that has so far dominated affinity determinations toward these targets." @default.
- W1897131549 created "2016-06-24" @default.
- W1897131549 creator A5003551748 @default.
- W1897131549 creator A5039007961 @default.
- W1897131549 creator A5063148334 @default.
- W1897131549 date "2015-09-01" @default.
- W1897131549 modified "2023-10-18" @default.
- W1897131549 title "MS Binding Assays for D<sub>1</sub>and D<sub>5</sub>Dopamine Receptors" @default.
- W1897131549 cites W1576980256 @default.
- W1897131549 cites W1976968057 @default.
- W1897131549 cites W1977869470 @default.
- W1897131549 cites W1984237245 @default.
- W1897131549 cites W2012753124 @default.
- W1897131549 cites W2017549779 @default.
- W1897131549 cites W2022151784 @default.
- W1897131549 cites W2022649017 @default.
- W1897131549 cites W2028701223 @default.
- W1897131549 cites W2039991333 @default.
- W1897131549 cites W2045231774 @default.
- W1897131549 cites W2055611087 @default.
- W1897131549 cites W2057392629 @default.
- W1897131549 cites W2071403847 @default.
- W1897131549 cites W2075744055 @default.
- W1897131549 cites W2078410528 @default.
- W1897131549 cites W2095348652 @default.
- W1897131549 cites W2107844475 @default.
- W1897131549 cites W2127273739 @default.
- W1897131549 cites W2127951177 @default.
- W1897131549 cites W2167162307 @default.
- W1897131549 cites W4230394365 @default.
- W1897131549 cites W4293247451 @default.
- W1897131549 doi "https://doi.org/10.1002/cmdc.201500355" @default.
- W1897131549 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26332653" @default.
- W1897131549 hasPublicationYear "2015" @default.
- W1897131549 type Work @default.
- W1897131549 sameAs 1897131549 @default.
- W1897131549 citedByCount "13" @default.
- W1897131549 countsByYear W18971315492017 @default.
- W1897131549 countsByYear W18971315492018 @default.
- W1897131549 countsByYear W18971315492019 @default.
- W1897131549 countsByYear W18971315492020 @default.
- W1897131549 countsByYear W18971315492021 @default.
- W1897131549 countsByYear W18971315492022 @default.
- W1897131549 countsByYear W18971315492023 @default.
- W1897131549 crossrefType "journal-article" @default.
- W1897131549 hasAuthorship W1897131549A5003551748 @default.
- W1897131549 hasAuthorship W1897131549A5039007961 @default.
- W1897131549 hasAuthorship W1897131549A5063148334 @default.
- W1897131549 hasConcept C107824862 @default.
- W1897131549 hasConcept C116569031 @default.
- W1897131549 hasConcept C120069818 @default.
- W1897131549 hasConcept C12554922 @default.
- W1897131549 hasConcept C169760540 @default.
- W1897131549 hasConcept C170493617 @default.
- W1897131549 hasConcept C185592680 @default.
- W1897131549 hasConcept C2909005528 @default.
- W1897131549 hasConcept C513476851 @default.
- W1897131549 hasConcept C55493867 @default.
- W1897131549 hasConcept C67847695 @default.
- W1897131549 hasConcept C71240020 @default.
- W1897131549 hasConcept C85196483 @default.
- W1897131549 hasConcept C86803240 @default.
- W1897131549 hasConceptScore W1897131549C107824862 @default.
- W1897131549 hasConceptScore W1897131549C116569031 @default.
- W1897131549 hasConceptScore W1897131549C120069818 @default.
- W1897131549 hasConceptScore W1897131549C12554922 @default.
- W1897131549 hasConceptScore W1897131549C169760540 @default.
- W1897131549 hasConceptScore W1897131549C170493617 @default.
- W1897131549 hasConceptScore W1897131549C185592680 @default.
- W1897131549 hasConceptScore W1897131549C2909005528 @default.
- W1897131549 hasConceptScore W1897131549C513476851 @default.
- W1897131549 hasConceptScore W1897131549C55493867 @default.
- W1897131549 hasConceptScore W1897131549C67847695 @default.
- W1897131549 hasConceptScore W1897131549C71240020 @default.
- W1897131549 hasConceptScore W1897131549C85196483 @default.
- W1897131549 hasConceptScore W1897131549C86803240 @default.
- W1897131549 hasIssue "11" @default.
- W1897131549 hasLocation W18971315491 @default.
- W1897131549 hasLocation W18971315492 @default.
- W1897131549 hasOpenAccess W1897131549 @default.
- W1897131549 hasPrimaryLocation W18971315491 @default.
- W1897131549 hasRelatedWork W1828383458 @default.
- W1897131549 hasRelatedWork W1897131549 @default.
- W1897131549 hasRelatedWork W1902906737 @default.
- W1897131549 hasRelatedWork W1973595242 @default.
- W1897131549 hasRelatedWork W1985444891 @default.
- W1897131549 hasRelatedWork W2178161815 @default.
- W1897131549 hasRelatedWork W2312486636 @default.
- W1897131549 hasRelatedWork W2411964334 @default.
- W1897131549 hasRelatedWork W2890983656 @default.
- W1897131549 hasRelatedWork W2089635480 @default.
- W1897131549 hasVolume "10" @default.
- W1897131549 isParatext "false" @default.
- W1897131549 isRetracted "false" @default.
- W1897131549 magId "1897131549" @default.
- W1897131549 workType "article" @default.