FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W189713698> ?p ?o ?g. }

• W189713698 endingPage "1701" @default.
• W189713698 startingPage "1694" @default.
• W189713698 abstract "A PCR assay for the detection of Bacillus cereus strains able to produce an emetic toxin (cereulide) was developed in this study based on a sequence-characterized amplified region (SCAR) derived from a random amplified polymorphic DNA (RAPD) fragment. One of the RAPD fragments generated was selected, cloned, and sequenced. A set of PCR primers was newly designed from the SCAR obtained (the sequence of the cloned RAPD fragment) and used in this assay. To determine the specificity of the assay, 30 different B. cereus strains, 8 other Bacillus strains (of six species), and 16 other non-Bacillus strains (from 16 genera) were tested. Results were positive for every emetic B. cereus strain and for only one nonemetic B. cereus strain. For all other bacterial strains, results were negative. Bacterial DNA for PCR was prepared by a simple procedure using Chelex 100 resin from the bacterial colony on the agar plate or from culture after growth in brain heart infusion medium. This PCR assay enabled us to detect the bacteria of emetic B. cereus grown on agar plates but not the bacteria of nonemetic B. cereus. To test this PCR assay for the monitoring of the emetic bacteria, 10 to 70 CFU of B. cereus DSM 4312 (emetic) per g of food was inoculated into several foods as an indicator, followed by a 7-h enrichment culture step. Because this PCR assay based on the SCAR derived from the RAPD fragment was able to detect bacterial cells, this assay should be useful for rapid and specific detection of emetic B. cereus." @default.
• W189713698 created "2016-06-24" @default.
• W189713698 creator A5024564529 @default.
• W189713698 creator A5041464056 @default.
• W189713698 creator A5059272915 @default.
• W189713698 creator A5060665974 @default.
• W189713698 creator A5068718188 @default.
• W189713698 creator A5069974247 @default.
• W189713698 creator A5082408580 @default.
• W189713698 date "2004-08-01" @default.
• W189713698 modified "2023-10-17" @default.
• W189713698 title "A PCR Assay Based on a Sequence-Characterized Amplified Region Marker for Detection of Emetic Bacillus cereus" @default.
• W189713698 cites W1511030774 @default.
• W189713698 cites W1548897202 @default.
• W189713698 cites W1551274364 @default.
• W189713698 cites W1673187150 @default.
• W189713698 cites W1801519214 @default.
• W189713698 cites W1982682630 @default.
• W189713698 cites W1987596588 @default.
• W189713698 cites W1991460093 @default.
• W189713698 cites W2019734042 @default.
• W189713698 cites W2024808550 @default.
• W189713698 cites W2030598579 @default.
• W189713698 cites W2040692141 @default.
• W189713698 cites W2052101309 @default.
• W189713698 cites W2052903624 @default.
• W189713698 cites W2065311355 @default.
• W189713698 cites W2077914268 @default.
• W189713698 cites W2081561143 @default.
• W189713698 cites W2095357583 @default.
• W189713698 cites W2111293220 @default.
• W189713698 cites W2128827593 @default.
• W189713698 cites W2134021339 @default.
• W189713698 cites W2318383157 @default.
• W189713698 cites W247176712 @default.
• W189713698 doi "https://doi.org/10.4315/0362-028x-67.8.1694" @default.
• W189713698 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15330536" @default.
• W189713698 hasPublicationYear "2004" @default.
• W189713698 type Work @default.
• W189713698 sameAs 189713698 @default.
• W189713698 citedByCount "24" @default.
• W189713698 countsByYear W1897136982013 @default.
• W189713698 countsByYear W1897136982015 @default.
• W189713698 countsByYear W1897136982016 @default.
• W189713698 countsByYear W1897136982017 @default.
• W189713698 countsByYear W1897136982022 @default.
• W189713698 crossrefType "journal-article" @default.
• W189713698 hasAuthorship W189713698A5024564529 @default.
• W189713698 hasAuthorship W189713698A5041464056 @default.
• W189713698 hasAuthorship W189713698A5059272915 @default.
• W189713698 hasAuthorship W189713698A5060665974 @default.
• W189713698 hasAuthorship W189713698A5068718188 @default.
• W189713698 hasAuthorship W189713698A5069974247 @default.
• W189713698 hasAuthorship W189713698A5082408580 @default.
• W189713698 hasBestOaLocation W1897136981 @default.
• W189713698 hasConcept C144024400 @default.
• W189713698 hasConcept C149923435 @default.
• W189713698 hasConcept C153911025 @default.
• W189713698 hasConcept C2777118263 @default.
• W189713698 hasConcept C2778660310 @default.
• W189713698 hasConcept C2779150676 @default.
• W189713698 hasConcept C2780373777 @default.
• W189713698 hasConcept C2908647359 @default.
• W189713698 hasConcept C523546767 @default.
• W189713698 hasConcept C54355233 @default.
• W189713698 hasConcept C62643968 @default.
• W189713698 hasConcept C81977670 @default.
• W189713698 hasConcept C84623179 @default.
• W189713698 hasConcept C86803240 @default.
• W189713698 hasConcept C89423630 @default.
• W189713698 hasConceptScore W189713698C144024400 @default.
• W189713698 hasConceptScore W189713698C149923435 @default.
• W189713698 hasConceptScore W189713698C153911025 @default.
• W189713698 hasConceptScore W189713698C2777118263 @default.
• W189713698 hasConceptScore W189713698C2778660310 @default.
• W189713698 hasConceptScore W189713698C2779150676 @default.
• W189713698 hasConceptScore W189713698C2780373777 @default.
• W189713698 hasConceptScore W189713698C2908647359 @default.
• W189713698 hasConceptScore W189713698C523546767 @default.
• W189713698 hasConceptScore W189713698C54355233 @default.
• W189713698 hasConceptScore W189713698C62643968 @default.
• W189713698 hasConceptScore W189713698C81977670 @default.
• W189713698 hasConceptScore W189713698C84623179 @default.
• W189713698 hasConceptScore W189713698C86803240 @default.
• W189713698 hasConceptScore W189713698C89423630 @default.
• W189713698 hasIssue "8" @default.
• W189713698 hasLocation W1897136981 @default.
• W189713698 hasLocation W1897136982 @default.
• W189713698 hasOpenAccess W189713698 @default.
• W189713698 hasPrimaryLocation W1897136981 @default.
• W189713698 hasRelatedWork W189713698 @default.
• W189713698 hasRelatedWork W1911906452 @default.
• W189713698 hasRelatedWork W2053724971 @default.
• W189713698 hasRelatedWork W2103229199 @default.
• W189713698 hasRelatedWork W2141556311 @default.
• W189713698 hasRelatedWork W2260686262 @default.
• W189713698 hasRelatedWork W2298986450 @default.
• W189713698 hasRelatedWork W3005999099 @default.

• next