Matches in SemOpenAlex for { <https://semopenalex.org/work/W1898445013> ?p ?o ?g. }
Showing items 1 to 95 of
95
with 100 items per page.
- W1898445013 endingPage "2210" @default.
- W1898445013 startingPage "2206" @default.
- W1898445013 abstract "Temperature-sensitive mutants of Actinobacillus pleuropneumoniae 4074, serotype 1, were isolated after treatment with nitrosoguanidine and enrichment with penicillin and D-cycloserine. Of the four temperature-sensitive mutants evaluated in mice, one (A-1) had a tight phenotype (i.e., it ceased replication immediately after transfer to the nonpermissive temperature [37 degrees C]) and three (1-2, 4-1, and 12-1) were coasters that continued replication for up to three generations after transfer to 37 degrees C. The reversion frequencies ranged from 10(-6) to 10(-9), and cutoff temperatures ranged from 33 to 35 degrees C. No major changes were detected in the biochemical profiles; agglutination reactions; electrophoretic profiles of the lipopolysaccharides, outer membrane proteins, and hemolysin proteins; hemolytic titers; or CAMP factor reactions of the mutants and the wild-type bacteria. Groups of 3- to 5-week-old, female ICR mice were immunized intranasally with three doses of 3.5 x 10(6) CFU of the mutants over 3 weeks and subsequently challenged intranasally with 5 50% lethal doses of the parental wild-type. Protection was induced by both the tight and the coaster mutants, with the 4-1 and 12-1 coasters eliciting greater protection (67 and 82%, respectively) than that induced by the A-1 tight mutant (57%). Intranasal immunization with both phenotypes induced serum antibody responses against the surface antigens and the hemolysin protein." @default.
- W1898445013 created "2016-06-24" @default.
- W1898445013 creator A5020865600 @default.
- W1898445013 creator A5048819746 @default.
- W1898445013 date "1997-06-01" @default.
- W1898445013 modified "2023-10-04" @default.
- W1898445013 title "Temperature-sensitive mutants of Actinobacillus pleuropneumoniae induce protection in mice" @default.
- W1898445013 cites W1837256871 @default.
- W1898445013 cites W1872353702 @default.
- W1898445013 cites W1885982018 @default.
- W1898445013 cites W1910204236 @default.
- W1898445013 cites W1910578357 @default.
- W1898445013 cites W1924462530 @default.
- W1898445013 cites W1965530211 @default.
- W1898445013 cites W1968834637 @default.
- W1898445013 cites W1968876388 @default.
- W1898445013 cites W1990020967 @default.
- W1898445013 cites W2009809804 @default.
- W1898445013 cites W2037649388 @default.
- W1898445013 cites W2037939302 @default.
- W1898445013 cites W2045141521 @default.
- W1898445013 cites W2050808860 @default.
- W1898445013 cites W2051191184 @default.
- W1898445013 cites W2100837269 @default.
- W1898445013 cites W2138163924 @default.
- W1898445013 cites W2154033714 @default.
- W1898445013 cites W2169396546 @default.
- W1898445013 doi "https://doi.org/10.1128/iai.65.6.2206-2210.1997" @default.
- W1898445013 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/175304" @default.
- W1898445013 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9169752" @default.
- W1898445013 hasPublicationYear "1997" @default.
- W1898445013 type Work @default.
- W1898445013 sameAs 1898445013 @default.
- W1898445013 citedByCount "3" @default.
- W1898445013 crossrefType "journal-article" @default.
- W1898445013 hasAuthorship W1898445013A5020865600 @default.
- W1898445013 hasAuthorship W1898445013A5048819746 @default.
- W1898445013 hasBestOaLocation W18984450131 @default.
- W1898445013 hasConcept C10389963 @default.
- W1898445013 hasConcept C104317684 @default.
- W1898445013 hasConcept C127716648 @default.
- W1898445013 hasConcept C136139683 @default.
- W1898445013 hasConcept C143065580 @default.
- W1898445013 hasConcept C143508840 @default.
- W1898445013 hasConcept C153911025 @default.
- W1898445013 hasConcept C159654299 @default.
- W1898445013 hasConcept C203014093 @default.
- W1898445013 hasConcept C207583985 @default.
- W1898445013 hasConcept C2780861368 @default.
- W1898445013 hasConcept C32611913 @default.
- W1898445013 hasConcept C54355233 @default.
- W1898445013 hasConcept C60987743 @default.
- W1898445013 hasConcept C70883133 @default.
- W1898445013 hasConcept C86803240 @default.
- W1898445013 hasConcept C89423630 @default.
- W1898445013 hasConceptScore W1898445013C10389963 @default.
- W1898445013 hasConceptScore W1898445013C104317684 @default.
- W1898445013 hasConceptScore W1898445013C127716648 @default.
- W1898445013 hasConceptScore W1898445013C136139683 @default.
- W1898445013 hasConceptScore W1898445013C143065580 @default.
- W1898445013 hasConceptScore W1898445013C143508840 @default.
- W1898445013 hasConceptScore W1898445013C153911025 @default.
- W1898445013 hasConceptScore W1898445013C159654299 @default.
- W1898445013 hasConceptScore W1898445013C203014093 @default.
- W1898445013 hasConceptScore W1898445013C207583985 @default.
- W1898445013 hasConceptScore W1898445013C2780861368 @default.
- W1898445013 hasConceptScore W1898445013C32611913 @default.
- W1898445013 hasConceptScore W1898445013C54355233 @default.
- W1898445013 hasConceptScore W1898445013C60987743 @default.
- W1898445013 hasConceptScore W1898445013C70883133 @default.
- W1898445013 hasConceptScore W1898445013C86803240 @default.
- W1898445013 hasConceptScore W1898445013C89423630 @default.
- W1898445013 hasIssue "6" @default.
- W1898445013 hasLocation W18984450131 @default.
- W1898445013 hasLocation W18984450132 @default.
- W1898445013 hasLocation W18984450133 @default.
- W1898445013 hasOpenAccess W1898445013 @default.
- W1898445013 hasPrimaryLocation W18984450131 @default.
- W1898445013 hasRelatedWork W1550659394 @default.
- W1898445013 hasRelatedWork W1566681514 @default.
- W1898445013 hasRelatedWork W1967456425 @default.
- W1898445013 hasRelatedWork W2019607410 @default.
- W1898445013 hasRelatedWork W2086988315 @default.
- W1898445013 hasRelatedWork W2095162143 @default.
- W1898445013 hasRelatedWork W2127130672 @default.
- W1898445013 hasRelatedWork W2188813829 @default.
- W1898445013 hasRelatedWork W2355476609 @default.
- W1898445013 hasRelatedWork W2372362125 @default.
- W1898445013 hasVolume "65" @default.
- W1898445013 isParatext "false" @default.
- W1898445013 isRetracted "false" @default.
- W1898445013 magId "1898445013" @default.
- W1898445013 workType "article" @default.