Matches in SemOpenAlex for { <https://semopenalex.org/work/W1898565573> ?p ?o ?g. }
Showing items 1 to 66 of
66
with 100 items per page.
- W1898565573 endingPage "406" @default.
- W1898565573 startingPage "405" @default.
- W1898565573 abstract "More than other analytical procedures, immunoassays have afforded a wealth of knowledge about biochemical physiology over the last 40 years. These assays have posed major analytical challenges, which most likely stem from the delicate balance between chemical equilibrium and our ever-increasing quest for speed of analysis, simplicity of procedure, and improved reliability. In attempts to enhance these criteria, we often face limitations imposed by the fundamental principles of thermodynamics: balancing chemical equilibrium, the underlying kinetics, and that ever-present variable, time.Cross-reactivity in competitive immunoassays can be measured by various methods, including 50% displacement, equal displacement, or the gradient approach (1). Typically, a cross-reactant is conceptually regarded as an interferent causing positive bias in the assay results. When an immunoassay is “rushed” (i.e., the signal is measured well before reaching equilibrium), the effect of the cross-reactant is enhanced, in essence increasing the positive bias (2). That is the expected phenomenon. The unexpected is the observation that cross-reactivity can also lead to suppression in recovery (3), i.e., a negative bias. In other words, the result obtained for a constant amount of the analyte in a sample is lower when the cross-reactant is present than when it is absent. This indicates that rigorous characterization of cross-reactivity requires studies in the presence of the analyte (1).Suppression of assay results induced by cross-reactivity was first described in Clinical Chemistry in 1996 for digoxin immunoassays (4). In retrospect, however, previous observations hinted at this problem. Kanan et al. (5) reported that in plasma obtained …" @default.
- W1898565573 created "2016-06-24" @default.
- W1898565573 creator A5012284116 @default.
- W1898565573 creator A5055767566 @default.
- W1898565573 date "2002-03-01" @default.
- W1898565573 modified "2023-09-23" @default.
- W1898565573 title "Unexpected Suppression of Immunoassay Results by Cross-Reactivity: Now a Demonstrated Cause for Concern" @default.
- W1898565573 cites W1888211986 @default.
- W1898565573 cites W1986473606 @default.
- W1898565573 cites W2092789247 @default.
- W1898565573 cites W2116111473 @default.
- W1898565573 doi "https://doi.org/10.1093/clinchem/48.3.405" @default.
- W1898565573 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11861431" @default.
- W1898565573 hasPublicationYear "2002" @default.
- W1898565573 type Work @default.
- W1898565573 sameAs 1898565573 @default.
- W1898565573 citedByCount "46" @default.
- W1898565573 countsByYear W18985655732012 @default.
- W1898565573 countsByYear W18985655732013 @default.
- W1898565573 countsByYear W18985655732014 @default.
- W1898565573 countsByYear W18985655732016 @default.
- W1898565573 countsByYear W18985655732017 @default.
- W1898565573 countsByYear W18985655732018 @default.
- W1898565573 countsByYear W18985655732019 @default.
- W1898565573 countsByYear W18985655732021 @default.
- W1898565573 countsByYear W18985655732022 @default.
- W1898565573 crossrefType "journal-article" @default.
- W1898565573 hasAuthorship W1898565573A5012284116 @default.
- W1898565573 hasAuthorship W1898565573A5055767566 @default.
- W1898565573 hasBestOaLocation W18985655731 @default.
- W1898565573 hasConcept C142724271 @default.
- W1898565573 hasConcept C159654299 @default.
- W1898565573 hasConcept C203014093 @default.
- W1898565573 hasConcept C204787440 @default.
- W1898565573 hasConcept C2776910235 @default.
- W1898565573 hasConcept C2780420688 @default.
- W1898565573 hasConcept C71924100 @default.
- W1898565573 hasConceptScore W1898565573C142724271 @default.
- W1898565573 hasConceptScore W1898565573C159654299 @default.
- W1898565573 hasConceptScore W1898565573C203014093 @default.
- W1898565573 hasConceptScore W1898565573C204787440 @default.
- W1898565573 hasConceptScore W1898565573C2776910235 @default.
- W1898565573 hasConceptScore W1898565573C2780420688 @default.
- W1898565573 hasConceptScore W1898565573C71924100 @default.
- W1898565573 hasIssue "3" @default.
- W1898565573 hasLocation W18985655731 @default.
- W1898565573 hasLocation W18985655732 @default.
- W1898565573 hasOpenAccess W1898565573 @default.
- W1898565573 hasPrimaryLocation W18985655731 @default.
- W1898565573 hasRelatedWork W1506200166 @default.
- W1898565573 hasRelatedWork W1995515455 @default.
- W1898565573 hasRelatedWork W2048182022 @default.
- W1898565573 hasRelatedWork W2080531066 @default.
- W1898565573 hasRelatedWork W2604872355 @default.
- W1898565573 hasRelatedWork W2748952813 @default.
- W1898565573 hasRelatedWork W2899084033 @default.
- W1898565573 hasRelatedWork W3031052312 @default.
- W1898565573 hasRelatedWork W3032375762 @default.
- W1898565573 hasRelatedWork W3108674512 @default.
- W1898565573 hasVolume "48" @default.
- W1898565573 isParatext "false" @default.
- W1898565573 isRetracted "false" @default.
- W1898565573 magId "1898565573" @default.
- W1898565573 workType "article" @default.