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• W1898751978 abstract "ABSTRACT A tris(2,2‐bipyridyl)ruthenium(II) (Ru(bpy) 3 2+ )‐based electrochemiluminescence (ECL) detection coupled with capillary electrophoresis (CE) method has been established for the sensitive determination of ephedrine for the first time. Under the optimized conditions [ECL detection at 1.15 V, 25 mmol/L phosphate buffer solution (PBS), pH 8.0, as running buffer, separation voltage 12.5 kV, 5 mmol/L Ru(bpy) 3 2+ with 60 mmol/L PBS, pH 8.5, in the detection cell] linear correlation ( r = 0.9987) between ECL intensity and ephedrine concentration was obtained in the range 6.0 × 10 –8 –6.0 × 10 –6 g/mL. The detection limit was 4.5 × 10 –9 g/mL (S:N = 3). The developed method was successfully applied to the analysis of ephedrine in human urine and the investigation of its interactions with three proteins, including bovine serum albumin (BSA), cytochrome C (Cyt‐C) and myoglobin (Mb). The number of binding sites and the binding constants between ephedrine and BSA, Cyt‐C and Mb were 8.52, 12.60, 10.66 and 1.55 × 10 4 mol/L, 6.58 × 10 3 mol/L and 1.59 × 10 4 mol/L, respectively. Copyright © 2011 John Wiley & Sons, Ltd." @default.
• W1898751978 created "2016-06-24" @default.
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• W1898751978 date "2011-08-01" @default.
• W1898751978 modified "2023-10-18" @default.
• W1898751978 title "Capillary electrophoresis coupled with end-column electrochemiluminescence for the determination of ephedrine in human urine, and a study of its interactions with three proteins" @default.
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• W1898751978 doi "https://doi.org/10.1002/bio.1336" @default.
• W1898751978 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21809433" @default.
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