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- W1898864698 abstract "Bovine Granulocutemacrophage colony stimulating factor (GM-CSF) gene was amplified by RT-PCR from total RNA isolated from LPS-stimulated peripheral blood T cells of cow.The PCR fragments was cloned into pGEM-T vector and sequenced.The gene contained about 430 bp and had 99.3 % (and 99.77 % at amino acid level) sequence identity with the published sequences.The gene was subcloned into pET32a vector and transformed into BL21 Escherichia coli cells.SDS-PAGE confirmed that the expressed protein was about 35 ku.MTT colorimetric assay and colony-forming test showed that the recombinant protein could induce bovine T lymphocytes to grow in vitro." @default.
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- W1898864698 date "2009-01-01" @default.
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- W1898864698 title "Prokaryotic expression and biological activity of bovine GM-CSF." @default.
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