FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1898981622> ?p ?o ?g. }

• W1898981622 endingPage "2234" @default.
• W1898981622 startingPage "2228" @default.
• W1898981622 abstract "Hepatitis delta virus (HDV) contains a circular RNA genome of 1.7 kb. HDV RNA replication is thought to proceed via a rolling-circle mechanism that is dependent on autocatalytic cleavage and ligation reactions. However, it has never been established that these ribozyme activities are indeed involved in HDV RNA replication. To investigate the possible biological significance of HDV RNA self-cleavage, we constructed several HDV dimer cDNAs containing single-base substitutions of the 3' nucleotide of the genomic and the antigenomic self-cleavage sites. These mutations were known to affect self-cleavage in vitro to various extents. The effects of these mutations on HDV RNA replication were examined in hepatic and nonhepatic cell lines. The results showed that all of the mutants which had lost the in vitro self-cleavage activity could not replicate. The only mutant which retained full cleavage activity replicated as efficiently as the wild-type RNA. Thus, this study established that self-cleavage activity is required for HDV RNA replication in cells. Interestingly, the level of HDV RNA detected in cells transfected with this replication-competent mutant and that detected in cells transfected with the wild-type construct were similar in COS-7 cells but vastly different in HepG2 and Huh-7 cells, suggesting that HDV RNA self-cleavage activity may be modulated by cell-specific factors. We also compared the effects of mutations when the primary transcripts of these constructs were of either genomic or antigenomic sense. In constructs which synthesize primary transcripts of genomic sense, all of the antigenomic self-cleavage mutants produced as much hepatitis delta antigen (HDAg) as did the wild-type construct, even in the absence of detectable HDV RNA replication, whereas the genomic self-cleavage mutants produced very little HDAg. These and other data suggest that (i) the primary HDV RNA transcripts of both genomic and antigenomic polarities must first be processed to serve as a template for HDV RNA transcription, (ii) efficient cleavage at the antigenomic self-cleavage site is not required for HDAg expression, and (iii) HDV RNA replication most likely occurs by a double-rolling-circle mechanism." @default.
• W1898981622 created "2016-06-24" @default.
• W1898981622 creator A5075023078 @default.
• W1898981622 creator A5076763316 @default.
• W1898981622 creator A5049915671 @default.
• W1898981622 date "1993-04-01" @default.
• W1898981622 modified "2023-10-01" @default.
• W1898981622 title "Replication of hepatitis delta virus RNA: effect of mutations of the autocatalytic cleavage sites" @default.
• W1898981622 cites W1490981311 @default.
• W1898981622 cites W1495704598 @default.
• W1898981622 cites W1502906266 @default.
• W1898981622 cites W1576679006 @default.
• W1898981622 cites W1605090496 @default.
• W1898981622 cites W1608621108 @default.
• W1898981622 cites W1678644792 @default.
• W1898981622 cites W1835568452 @default.
• W1898981622 cites W1955850947 @default.
• W1898981622 cites W1968642409 @default.
• W1898981622 cites W1998961564 @default.
• W1898981622 cites W1999606717 @default.
• W1898981622 cites W1999631927 @default.
• W1898981622 cites W2005064067 @default.
• W1898981622 cites W2011412411 @default.
• W1898981622 cites W2013659134 @default.
• W1898981622 cites W2017895919 @default.
• W1898981622 cites W2018802169 @default.
• W1898981622 cites W2030156849 @default.
• W1898981622 cites W2035991334 @default.
• W1898981622 cites W2044480725 @default.
• W1898981622 cites W2051391596 @default.
• W1898981622 cites W2052336952 @default.
• W1898981622 cites W2060505477 @default.
• W1898981622 cites W2068797168 @default.
• W1898981622 cites W2134812217 @default.
• W1898981622 cites W2138336436 @default.
• W1898981622 cites W2163414819 @default.
• W1898981622 cites W2171760409 @default.
• W1898981622 cites W2325021964 @default.
• W1898981622 doi "https://doi.org/10.1128/jvi.67.4.2228-2234.1993" @default.
• W1898981622 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/240348" @default.
• W1898981622 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8445730" @default.
• W1898981622 hasPublicationYear "1993" @default.
• W1898981622 type Work @default.
• W1898981622 sameAs 1898981622 @default.
• W1898981622 citedByCount "74" @default.
• W1898981622 countsByYear W18989816222014 @default.
• W1898981622 countsByYear W18989816222015 @default.
• W1898981622 countsByYear W18989816222016 @default.
• W1898981622 countsByYear W18989816222017 @default.
• W1898981622 countsByYear W18989816222018 @default.
• W1898981622 countsByYear W18989816222020 @default.
• W1898981622 countsByYear W18989816222021 @default.
• W1898981622 countsByYear W18989816222022 @default.
• W1898981622 crossrefType "journal-article" @default.
• W1898981622 hasAuthorship W1898981622A5049915671 @default.
• W1898981622 hasAuthorship W1898981622A5075023078 @default.
• W1898981622 hasAuthorship W1898981622A5076763316 @default.
• W1898981622 hasBestOaLocation W18989816221 @default.
• W1898981622 hasConcept C104317684 @default.
• W1898981622 hasConcept C140704245 @default.
• W1898981622 hasConcept C143065580 @default.
• W1898981622 hasConcept C151730666 @default.
• W1898981622 hasConcept C153911025 @default.
• W1898981622 hasConcept C159047783 @default.
• W1898981622 hasConcept C175156509 @default.
• W1898981622 hasConcept C2522874641 @default.
• W1898981622 hasConcept C41258723 @default.
• W1898981622 hasConcept C43369102 @default.
• W1898981622 hasConcept C54355233 @default.
• W1898981622 hasConcept C67705224 @default.
• W1898981622 hasConcept C76346623 @default.
• W1898981622 hasConcept C86803240 @default.
• W1898981622 hasConceptScore W1898981622C104317684 @default.
• W1898981622 hasConceptScore W1898981622C140704245 @default.
• W1898981622 hasConceptScore W1898981622C143065580 @default.
• W1898981622 hasConceptScore W1898981622C151730666 @default.
• W1898981622 hasConceptScore W1898981622C153911025 @default.
• W1898981622 hasConceptScore W1898981622C159047783 @default.
• W1898981622 hasConceptScore W1898981622C175156509 @default.
• W1898981622 hasConceptScore W1898981622C2522874641 @default.
• W1898981622 hasConceptScore W1898981622C41258723 @default.
• W1898981622 hasConceptScore W1898981622C43369102 @default.
• W1898981622 hasConceptScore W1898981622C54355233 @default.
• W1898981622 hasConceptScore W1898981622C67705224 @default.
• W1898981622 hasConceptScore W1898981622C76346623 @default.
• W1898981622 hasConceptScore W1898981622C86803240 @default.
• W1898981622 hasIssue "4" @default.
• W1898981622 hasLocation W18989816221 @default.
• W1898981622 hasLocation W18989816222 @default.
• W1898981622 hasLocation W18989816223 @default.
• W1898981622 hasLocation W18989816224 @default.
• W1898981622 hasOpenAccess W1898981622 @default.
• W1898981622 hasPrimaryLocation W18989816221 @default.
• W1898981622 hasRelatedWork W1979364556 @default.
• W1898981622 hasRelatedWork W2001396871 @default.
• W1898981622 hasRelatedWork W2002484773 @default.
• W1898981622 hasRelatedWork W2053542463 @default.
• W1898981622 hasRelatedWork W2086632997 @default.

• next