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• W1899206873 endingPage "1121" @default.
• W1899206873 startingPage "1115" @default.
• W1899206873 abstract "Pseudomonas aeruginosa strains isolated from patients with cystic fibrosis commonly produce a capsule-like exopolysaccharide called alginate. The alginate-producing (Alg+) phenotype results in a mucoid colony morphology and is an unstable trait. A mutant of P. aeruginosa FRD (a cystic fibrosis isolate) was obtained which was temperature sensitive for alginate production ( Algts ). At elevated growth temperatures (41 degrees C), no alginate was detected in culture supernatants of the Algts mutant, but yields of alginate increased as the temperature of incubation was reduced. The mutation responsible for the Algts phenotype, alg-50(Ts), has been mapped to a region of the FRD chromosome closely linked to trp-2. The alg-50(Ts) marker did not map near the met-l-linked chromosomal mutations responsible for the instability of the Alg+ phenotype. A broad host range cosmid cloning system based upon derivatives of plasmid RK2 was used to construct a P. aeruginosa clone bank. After transfer of the clone bank to the Algts mutant, hybrid plasmids were obtained which complemented the Algts defect. Deletion mapping of the original 20.3 kilobases of P. aeruginosa DNA cloned showed that a 4.7-kilobase fragment would complement the alg-50(Ts) mutation." @default.
• W1899206873 created "2016-06-24" @default.
• W1899206873 creator A5032258415 @default.
• W1899206873 creator A5055515370 @default.
• W1899206873 date "1984-06-01" @default.
• W1899206873 modified "2023-10-13" @default.
• W1899206873 title "Cloning and expression in Pseudomonas aeruginosa of a gene involved in the production of alginate" @default.
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• W1899206873 doi "https://doi.org/10.1128/jb.158.3.1115-1121.1984" @default.
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