FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1899390263> ?p ?o ?g. }

• W1899390263 endingPage "4195" @default.
• W1899390263 startingPage "4189" @default.
• W1899390263 abstract "The hutC gene in Pseudomonas putida encodes a repressor protein that negatively regulates the expression of all hut genes. We have overexpressed this cloned hutC gene in Escherichia coli to identify P. putida hut regions that could specifically bind the repressor. Ten restriction fragments, some of which were partially overlapping and spanned the coding portions of the P. putida hut region, were labeled and tested for their ability to recognize repressor in a filter binding assay. This procedure identified three binding sites, thus supporting previous indications that there were multiple operons. A 1.0-kilobase-pair SalI restriction fragment contained the operator region for the hutUHIG operon, whereas a 1.9-kilobase-pair SmaI fragment contained the hutF operator. A 2.9-kilobase-pair XhoI segment appeared to contain the third operator, corresponding to a separate and perhaps little used control region for hutG expression only. The addition of urocanate, the normal inducer, caused dissociation of all operator-repressor complexes, whereas N-formylglutamate, capable of specifically inducing expression of the hutG gene, inhibited binding only of repressor to fragments containing that gene. Formylglutamate did not affect the action of urocanate on the repressor-hutUHIG operator complex, indicating that it binds to a site separate from urocanate on the repressor. DNA footprinting and gel retardation analyses were used to locate more precisely the operator for the hutUHIG operon. A roughly 40-base-pair portion was identified which contained a 16-base-pair region of dyad symmetry located near the transcription initiation site for this operon." @default.
• W1899390263 created "2016-06-24" @default.
• W1899390263 creator A5022588100 @default.
• W1899390263 creator A5033763954 @default.
• W1899390263 creator A5071730352 @default.
• W1899390263 date "1989-08-01" @default.
• W1899390263 modified "2023-10-16" @default.
• W1899390263 title "Identification of multiple repressor recognition sites in the hut system of Pseudomonas putida" @default.
• W1899390263 cites W1519804782 @default.
• W1899390263 cites W1639233254 @default.
• W1899390263 cites W1644846887 @default.
• W1899390263 cites W1898882187 @default.
• W1899390263 cites W1977528037 @default.
• W1899390263 cites W1978439483 @default.
• W1899390263 cites W1997136132 @default.
• W1899390263 cites W2033529844 @default.
• W1899390263 cites W2070174251 @default.
• W1899390263 cites W2110088230 @default.
• W1899390263 cites W2128635872 @default.
• W1899390263 cites W2146249297 @default.
• W1899390263 cites W2403230530 @default.
• W1899390263 cites W4293247451 @default.
• W1899390263 cites W967642706 @default.
• W1899390263 doi "https://doi.org/10.1128/jb.171.8.4189-4195.1989" @default.
• W1899390263 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/210189" @default.
• W1899390263 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2666390" @default.
• W1899390263 hasPublicationYear "1989" @default.
• W1899390263 type Work @default.
• W1899390263 sameAs 1899390263 @default.
• W1899390263 citedByCount "37" @default.
• W1899390263 countsByYear W18993902632012 @default.
• W1899390263 countsByYear W18993902632013 @default.
• W1899390263 countsByYear W18993902632014 @default.
• W1899390263 countsByYear W18993902632015 @default.
• W1899390263 countsByYear W18993902632016 @default.
• W1899390263 countsByYear W18993902632020 @default.
• W1899390263 crossrefType "journal-article" @default.
• W1899390263 hasAuthorship W1899390263A5022588100 @default.
• W1899390263 hasAuthorship W1899390263A5033763954 @default.
• W1899390263 hasAuthorship W1899390263A5071730352 @default.
• W1899390263 hasBestOaLocation W18993902632 @default.
• W1899390263 hasConcept C101425835 @default.
• W1899390263 hasConcept C104317684 @default.
• W1899390263 hasConcept C128319531 @default.
• W1899390263 hasConcept C150194340 @default.
• W1899390263 hasConcept C153911025 @default.
• W1899390263 hasConcept C158448853 @default.
• W1899390263 hasConcept C17020691 @default.
• W1899390263 hasConcept C170344550 @default.
• W1899390263 hasConcept C185581394 @default.
• W1899390263 hasConcept C203075996 @default.
• W1899390263 hasConcept C2778910516 @default.
• W1899390263 hasConcept C2780543182 @default.
• W1899390263 hasConcept C54355233 @default.
• W1899390263 hasConcept C547475151 @default.
• W1899390263 hasConcept C58301748 @default.
• W1899390263 hasConcept C86803240 @default.
• W1899390263 hasConceptScore W1899390263C101425835 @default.
• W1899390263 hasConceptScore W1899390263C104317684 @default.
• W1899390263 hasConceptScore W1899390263C128319531 @default.
• W1899390263 hasConceptScore W1899390263C150194340 @default.
• W1899390263 hasConceptScore W1899390263C153911025 @default.
• W1899390263 hasConceptScore W1899390263C158448853 @default.
• W1899390263 hasConceptScore W1899390263C17020691 @default.
• W1899390263 hasConceptScore W1899390263C170344550 @default.
• W1899390263 hasConceptScore W1899390263C185581394 @default.
• W1899390263 hasConceptScore W1899390263C203075996 @default.
• W1899390263 hasConceptScore W1899390263C2778910516 @default.
• W1899390263 hasConceptScore W1899390263C2780543182 @default.
• W1899390263 hasConceptScore W1899390263C54355233 @default.
• W1899390263 hasConceptScore W1899390263C547475151 @default.
• W1899390263 hasConceptScore W1899390263C58301748 @default.
• W1899390263 hasConceptScore W1899390263C86803240 @default.
• W1899390263 hasIssue "8" @default.
• W1899390263 hasLocation W18993902631 @default.
• W1899390263 hasLocation W18993902632 @default.
• W1899390263 hasLocation W18993902633 @default.
• W1899390263 hasLocation W18993902634 @default.
• W1899390263 hasOpenAccess W1899390263 @default.
• W1899390263 hasPrimaryLocation W18993902631 @default.
• W1899390263 hasRelatedWork W1492710178 @default.
• W1899390263 hasRelatedWork W1670843076 @default.
• W1899390263 hasRelatedWork W1899390263 @default.
• W1899390263 hasRelatedWork W2018841288 @default.
• W1899390263 hasRelatedWork W2045725779 @default.
• W1899390263 hasRelatedWork W2056573287 @default.
• W1899390263 hasRelatedWork W2134174762 @default.
• W1899390263 hasRelatedWork W2160587790 @default.
• W1899390263 hasRelatedWork W417396621 @default.
• W1899390263 hasRelatedWork W80365941 @default.
• W1899390263 hasVolume "171" @default.
• W1899390263 isParatext "false" @default.
• W1899390263 isRetracted "false" @default.
• W1899390263 magId "1899390263" @default.
• W1899390263 workType "article" @default.