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- W189958260 abstract "Flow cytometry (FC) is a powerful technique for the rapid analysis of single cells in a mixture, by means of light-scattering and fluorescence measurements. The power of this method in biotechnology lies both in the possibility of determining a wide range of cell parameters at the single level, and in the ability to obtain information about their distribution within cell populations, thus providing valuable information for bioprocess design and control. The first part of this article will be devoted to describing the basic aspects of multicolor FC, the different strategies used for cell detection, and the cellular target sites and fluorescent probes currently used in cell assays. Next, an in-depth revision concerning the most recent FC applications related to cell analysis and quantification (especially both bacteria and yeast) in food, beverages, and pharmaceutical industries, and in natural environment and water systems is presented. This article seeks to highlight the advantages of this technique in microbial fermentations monitoring and control, as well as in the development of more accurate kinetic models directed to bioprocesses optimization. Finally, an overview of some commercial FC devices is presented, along with a discussion of the progress and advantages of the method for bioprocesses online monitoring." @default.
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- W189958260 date "2011-01-01" @default.
- W189958260 modified "2023-10-16" @default.
- W189958260 title "Flow Cytometry" @default.
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- W189958260 doi "https://doi.org/10.1016/b978-0-08-088504-9.00448-7" @default.
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