Matches in SemOpenAlex for { <https://semopenalex.org/work/W1900516489> ?p ?o ?g. }
Showing items 1 to 93 of
93
with 100 items per page.
- W1900516489 endingPage "709" @default.
- W1900516489 startingPage "703" @default.
- W1900516489 abstract "Proteins extracted with 6 M guanidine at 90 degrees C from conidia (asexual spores) of Neurospora crassa contained ca. 25% more total protein thiol and a fivefold-higher content of disulfide bonds than proteins extracted from mycelia, as determined by labeling with iodo[14C]acetic acid. The total thiol content was 88 mumol/g of protein in conidia and 70 mumol/g of protein in mycelia. The level of protein disulfide was 18.5 mumol/g of protein in conidia and 3.5 mumol/g of protein in mycelia, by the iodo[14C]acetic acid labeling method. Confirmatory results were obtained with 5'5-dithio-bis-2-nitrobenzoic acid titration of protein thiol groups in 1% sodium dodecyl sulfate as well as by amino acid analysis of cysteic acid derivatives. Buffer-extracted proteins from conidia, but not mycelia, were found to contain enriched levels of protein thiols and disulfides per gram of protein as compared with guanidine hydrochloride extracts. It was demonstrated that the high disulfide content of crude conidial extracts was not due to measurable levels of mixed disulfides formed between protein sulfhydryl groups and cysteine. During germination of the conidia, the high disulfide levels of the conidial proteins remained constant. These data suggest that, unlike the disulfides of glutathione, the bulk of conidial protein disulfides were not reduced, excreted, or extensively degraded during germination." @default.
- W1900516489 created "2016-06-24" @default.
- W1900516489 creator A5065841321 @default.
- W1900516489 creator A5072454263 @default.
- W1900516489 creator A5078574177 @default.
- W1900516489 date "1983-11-01" @default.
- W1900516489 modified "2023-09-23" @default.
- W1900516489 title "Levels of sulfhydryls and disulfides in proteins from Neurospora crassa conidia and mycelia" @default.
- W1900516489 cites W1093746230 @default.
- W1900516489 cites W1535536969 @default.
- W1900516489 cites W1535590895 @default.
- W1900516489 cites W1539271460 @default.
- W1900516489 cites W1540075853 @default.
- W1900516489 cites W160146436 @default.
- W1900516489 cites W1651614514 @default.
- W1900516489 cites W1775749144 @default.
- W1900516489 cites W1813333262 @default.
- W1900516489 cites W1815037918 @default.
- W1900516489 cites W1830800654 @default.
- W1900516489 cites W1977278270 @default.
- W1900516489 cites W2040533353 @default.
- W1900516489 cites W2140647347 @default.
- W1900516489 doi "https://doi.org/10.1128/jb.156.2.703-709.1983" @default.
- W1900516489 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/217886" @default.
- W1900516489 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6226648" @default.
- W1900516489 hasPublicationYear "1983" @default.
- W1900516489 type Work @default.
- W1900516489 sameAs 1900516489 @default.
- W1900516489 citedByCount "3" @default.
- W1900516489 crossrefType "journal-article" @default.
- W1900516489 hasAuthorship W1900516489A5065841321 @default.
- W1900516489 hasAuthorship W1900516489A5072454263 @default.
- W1900516489 hasAuthorship W1900516489A5078574177 @default.
- W1900516489 hasBestOaLocation W19005164891 @default.
- W1900516489 hasConcept C104317684 @default.
- W1900516489 hasConcept C133479454 @default.
- W1900516489 hasConcept C143065580 @default.
- W1900516489 hasConcept C181199279 @default.
- W1900516489 hasConcept C185592680 @default.
- W1900516489 hasConcept C2776515853 @default.
- W1900516489 hasConcept C2776673659 @default.
- W1900516489 hasConcept C2776739539 @default.
- W1900516489 hasConcept C2776769606 @default.
- W1900516489 hasConcept C2779201268 @default.
- W1900516489 hasConcept C2779463914 @default.
- W1900516489 hasConcept C2780197888 @default.
- W1900516489 hasConcept C43143990 @default.
- W1900516489 hasConcept C515207424 @default.
- W1900516489 hasConcept C538909803 @default.
- W1900516489 hasConcept C55493867 @default.
- W1900516489 hasConcept C59822182 @default.
- W1900516489 hasConcept C86803240 @default.
- W1900516489 hasConceptScore W1900516489C104317684 @default.
- W1900516489 hasConceptScore W1900516489C133479454 @default.
- W1900516489 hasConceptScore W1900516489C143065580 @default.
- W1900516489 hasConceptScore W1900516489C181199279 @default.
- W1900516489 hasConceptScore W1900516489C185592680 @default.
- W1900516489 hasConceptScore W1900516489C2776515853 @default.
- W1900516489 hasConceptScore W1900516489C2776673659 @default.
- W1900516489 hasConceptScore W1900516489C2776739539 @default.
- W1900516489 hasConceptScore W1900516489C2776769606 @default.
- W1900516489 hasConceptScore W1900516489C2779201268 @default.
- W1900516489 hasConceptScore W1900516489C2779463914 @default.
- W1900516489 hasConceptScore W1900516489C2780197888 @default.
- W1900516489 hasConceptScore W1900516489C43143990 @default.
- W1900516489 hasConceptScore W1900516489C515207424 @default.
- W1900516489 hasConceptScore W1900516489C538909803 @default.
- W1900516489 hasConceptScore W1900516489C55493867 @default.
- W1900516489 hasConceptScore W1900516489C59822182 @default.
- W1900516489 hasConceptScore W1900516489C86803240 @default.
- W1900516489 hasIssue "2" @default.
- W1900516489 hasLocation W19005164891 @default.
- W1900516489 hasLocation W19005164892 @default.
- W1900516489 hasLocation W19005164893 @default.
- W1900516489 hasOpenAccess W1900516489 @default.
- W1900516489 hasPrimaryLocation W19005164891 @default.
- W1900516489 hasRelatedWork W1499965780 @default.
- W1900516489 hasRelatedWork W1607200279 @default.
- W1900516489 hasRelatedWork W1674514551 @default.
- W1900516489 hasRelatedWork W1900516489 @default.
- W1900516489 hasRelatedWork W1997801720 @default.
- W1900516489 hasRelatedWork W2000943360 @default.
- W1900516489 hasRelatedWork W2014382618 @default.
- W1900516489 hasRelatedWork W2091219986 @default.
- W1900516489 hasRelatedWork W2121031114 @default.
- W1900516489 hasRelatedWork W2775091051 @default.
- W1900516489 hasVolume "156" @default.
- W1900516489 isParatext "false" @default.
- W1900516489 isRetracted "false" @default.
- W1900516489 magId "1900516489" @default.
- W1900516489 workType "article" @default.