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• W1900543109 startingPage "881" @default.
• W1900543109 abstract "Colchicine-resistant Chinese hamster ovary (CHO) cell mutants whose resistance results from reduced drug permeability have been isolated previously in our laboratories. This reduced permeability affects a wide range of unrelated drugs, resulting in the mutants displaying a multiple drug resistance phenotype. A 170,000-dalton cell surface glycoprotein (P-glycoprotein) was identified, and its expression appears to correlate with the degree of resistance. In this study we were able to confer the multiple drug resistance phenotype on sensitive mouse L cells by DNA-mediated gene transfer of DNA obtained from the colchicine-resistant mutants. P-glycoprotein was detected in plasma membranes of these DNA transformants by staining with an antiserum raised against membranes of mutant CHO cells. These results are consistent with a causal relationship between P-glycoprotein expression and the multiple drug resistance phenotype." @default.
• W1900543109 created "2016-06-24" @default.
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• W1900543109 date "1982-08-01" @default.
• W1900543109 modified "2023-10-16" @default.
• W1900543109 title "DNA-mediated transfer of multiple drug resistance and plasma membrane glycoprotein expression." @default.
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• W1900543109 doi "https://doi.org/10.1128/mcb.2.8.881" @default.
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