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- W1900609241 abstract "Effect of the Polyethyleneimine in the Activity Catalytic of the horseradish peroxidase Immobilized on Gold Electrodes Modified with a Self-assembled Monolayer of Thiols (SAMs). Studies were conducted by cyclic voltammetry (CV) to investigate the effect of the polymer polyethyleneimine (PEI) in the electrochemical reversibility of the mediator thionine and thus the catalytic activity of the enzyme horseradish peroxidase of recombinant HRP-NHis (horseradish peroxidase to the has been added to a chain of six histidine in the extreme N-terminal protein). This self produced monolayers of thiols (SAMS) on gold electrodes, with chemical modifications obtained through successive stages in the solid phase of the electrode. The gold electrodes were modified with monolayer SAM-TOA-(ANTA/DADOO)-Co 2+ (SAM: self-assembled monolayers of thiols, TOA: dithioctic acid, ANTA: nitrilotriacetic acid, DADOO: 1,8-diamino-3,6-dioxaoctane). The results showed that the presence of the polymer improves the electrochemical reversibility of the mediator to endure catalytic currents as high as those that are obtained with molar ratios ANTA:DADOO 10:1 in the absence of PEI, and improve the response voltammetric obtained." @default.
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- W1900609241 date "2009-05-01" @default.
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- W1900609241 title "Efecto de la polietilenimina en la actividad catalítica de la peroxidasa de rábano (horseradish peroxidase) inmovilizada en electrodos de oro modificados con monocapas autoensambladas de tioles (SAMs)." @default.
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